ArticlePloS one2016
A Study of Alterations in DNA Epigenetic Modifications (5mC and 5hmC) and Gene Expression Influenced by Simulated Microgravity in Human Lymphoblastoid Cells.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 39 citations in OpenAlex.
- A systematic review of microgravity on reproductive systems: Implications for space biology and human health.iScience · 2026Review
- Epigenetics in Space: The Dynamic Cellular Response.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Epigenomic profiling of immune cell subtypes reveals H3K27ac-marked stress signatures after long-duration spaceflight.Scientific reports · 2025Article
- A comprehensive guide to genomeZhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2025Review
- Aging and Altered Gravity: A Cellular Perspective.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Review
- Omics Studies of Specialized Cells and Stem Cells under Microgravity Conditions.International journal of molecular sciences · 2024Review
- Aging and putative frailty biomarkers are altered by spaceflight.Scientific reports · 2024Article
- Omics Studies of Tumor Cells under Microgravity Conditions.International journal of molecular sciences · 2024Review
- Article
- The Oxidative Phosphorylation and Cytoskeleton Proteins of Mouse Ovaries after 96 Hours of Hindlimb Suspension.Life (Basel, Switzerland) · 2023Article
- Potential of natural medicines for treatment of osteoporosis: a narrative review.Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan · 2023Review
- Genomic Changes Driven by Radiation-Induced DNA Damage and Microgravity in Human Cells.International journal of molecular sciences · 2021Review
- Analysis of the effects of magnetic levitation to simulate microgravity environment on the Arp2/3 complex pathway in macrophage.Journal of biological physics · 2021Article
- Rapid Transient Transcriptional Adaptation to Hypergravity in Jurkat T Cells Revealed by Comparative Analysis of Microarray and RNA-Seq Data.International journal of molecular sciences · 2021Article
- Gravitational Influence on Human Living Systems and the Evolution of Species on Earth.Molecules (Basel, Switzerland) · 2021Review
- No evidence for DNAScience advances · 2020Article
- Testes and duct deferens of mice during space flight: cytoskeleton structure, sperm-specific proteins and epigenetic events.Scientific reports · 2019Article
- DNA Methylation of Mouse Testes, Cardiac and Lung Tissue During Long-Term Microgravity Simulation.Scientific reports · 2019Article
- [Mitochondrial DNA hydroxymethylation level in the cerebral cortex of neonatal rats with hypoxic-ischemic brain damage].Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics · 2019Article
- X-ray irradiation induces subtle changes in the genome-wide distribution of DNA hydroxymethylation with opposing trends in genic and intergenic regions.Epigenetics · 2019Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Cells alter their gene expression in response to exposure to various environmental changes. Epigenetic mechanisms such as DNA methylation are believed to regulate the alterations in gene expression patterns. In vitro and in vivo studies have documented changes in cellular proliferation, cytoskeletal remodeling, signal transduction, bone mineralization and immune deficiency under the influence of microgravity conditions experienced in space. However microgravity induced changes in the epigenome have not been well characterized. In this study we have used Next-generation Sequencing (NGS) to profile ground-based "simulated" microgravity induced changes on DNA methylation (5-methylcytosine or 5mC), hydroxymethylation (5-hydroxymethylcytosine or 5hmC), and simultaneous gene expression in cultured human lymphoblastoid cells. Our results indicate that simulated microgravity induced alterations in the methylome (~60% of the differentially methylated regions or DMRs are hypomethylated and ~92% of the differentially hydroxymethylated regions or DHMRs are hyperhydroxymethylated). Simulated microgravity also induced differential expression in 370 transcripts that were associated with crucial biological processes such as oxidative stress response, carbohydrate metabolism and regulation of transcription. While we were not able to obtain any global trend correlating the changes of methylation/ hydroxylation with gene expression, we have been able to profile the simulated microgravity induced changes of 5mC over some of the differentially expressed genes that includes five genes undergoing differential methylation over their promoters and twenty five genes undergoing differential methylation over their gene-bodies. To the best of our knowledge, this is the first NGS-based study to profile epigenomic patterns induced by short time exposure of simulated microgravity and we believe that our findings can be a valuable resource for future explorations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.