Evidence map›Paper›PMID 26820518›Full record

ArticleInternational urology and nephrology2016

Effects of adipose-derived stem cells plus insulin on erectile function in streptozotocin-induced diabetic rats.

Feng Zhou, Yu Hui, Yongde Xu, Hongen Lei, Bicheng Yang, Ruili Guan, Zhezhu Gao, Zhongcheng Xin, Jianquan Hou

Abstract read
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In one paragraph

Article in International urology and nephrology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.

  1. Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Feng ZhouDepartment of Urology, First Affiliated Hospital of Soochow University, Soochow University, No. 188, Shizi Street, Gusu District, Suzhou, 215006, China.
Yu HuiDepartment of Urology, First Affiliated Hospital of Soochow University, Soochow University, No. 188, Shizi Street, Gusu District, Suzhou, 215006, China.
Yongde XuMolecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, No. A59, Di'anmen West Street, Xicheng District, Beijing, 100034, China.
Hongen LeiMolecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, No. A59, Di'anmen West Street, Xicheng District, Beijing, 100034, China.
Bicheng YangMolecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, No. A59, Di'anmen West Street, Xicheng District, Beijing, 100034, China.
Ruili GuanMolecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, No. A59, Di'anmen West Street, Xicheng District, Beijing, 100034, China.
Zhezhu GaoMolecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, No. A59, Di'anmen West Street, Xicheng District, Beijing, 100034, China.
Zhongcheng XinMolecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, No. A59, Di'anmen West Street, Xicheng District, Beijing, 100034, China. xinzc@bjmu.edu.cn.
Jianquan HouDepartment of Urology, First Affiliated Hospital of Soochow University, Soochow University, No. 188, Shizi Street, Gusu District, Suzhou, 215006, China. hjq0192@foxmail.com.
Peking University First Hospital · CNFirst Affiliated Hospital of Soochow University · CNPeking University · CNSoochow University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeErectile dysfunction (ED) is a distressing complication in men with diabetes mellitus (DM). This study aimed to investigate the effects of adipose-derived stem cells (ADSCs) plus insulin on ED in streptozotocin (STZ)-induced diabetic rats.

methodsForty-five eight-week-old male Sprague-Dawley rats received intraperitoneal injection of STZ (60 mg/kg). Eight weeks after the induction, the determined diabetic rats were randomly distributed into four groups: rats in DM + PBS group received a one-time intracavernous (IC) injection of phosphate-buffered saline (PBS) solution, DM + ADSCs group received IC injection of ADSCs, DM + Insulin group received subcutaneous injection of neutral protamine Hagedorn twice a day, and DM + ADSCs + Insulin group received both ADSCs and neutral protamine Hagedorn treatments. Another 10 normal rats were served as control group and received IC injection of PBS. Four weeks after the treatments, intracavernous pressure, histopathological changes in penis, functional proteins of ADSCs, and penis were measured.

resultsWe found that ADSCs expressed vascular endothelial growth factor, TIMP metallopeptidase inhibitor 1 (TIMP-1), and lipopolysaccharide-inducible CXC chemokine (LIX). ADSC injection partially restored cavernous endothelium and smooth muscle contents and nNOS-positive nerves, and reduced apoptosis in penis compared with PBS-treated diabetic rats. Insulin treatment could further modulate inflammatory response and reduce advanced glycation end-product accumulation in penis.

conclusionsBetter than single therapy, ADSCs combined with insulin ameliorate ED and pathological changes in diabetic rats to near-normal levels.

Indexed as

Stem Cell TransplantationAdipose TissueAnimalsApoptosisChemokine CXCL5Combined Modality TherapyDiabetes Mellitus, ExperimentalErectile DysfunctionGlycation End Products, AdvancedHypoglycemic AgentsInflammationInsulin, IsophaneMaleNitric Oxide Synthase Type IPenisPeripheral NervesAger protein, ratChemokine CXCL5Glycation End Products, AdvancedHypoglycemic AgentsInsulin, IsophaneNitric Oxide Synthase Type IReceptor for Advanced Glycation End ProductsTIMP1 protein, ratTissue Inhibitor of Metalloproteinase-1Vascular Endothelial Growth Factor AAdipose-derived stem cellsAGEsDiabetesErectile dysfunctionInsulinVEGF

Identifiers

PMID26820518
OpenAlexW2307839435

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.