ArticlePeerJ2016
Expression patterns of cysteine peptidase genes across the Tribolium castaneum life cycle provide clues to biological function.
Article in PeerJ, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Recapitulation of the embryonic transcriptional program in holometabolous insect pupae.Scientific reports · 2022Article
- Recombinant Cathepsin L ofInternational journal of molecular sciences · 2022Article
- The Genome ofGenes · 2022Article
- Brown marmorated stink bug, Halyomorpha halys (Stål), genome: putative underpinnings of polyphagy, insecticide resistance potential and biology of a top worldwide pest.BMC genomics · 2020Article
- Proteolytic profile of larval developmental stages of Penaeus vannamei: An activity and mRNA expression approach.PloS one · 2020Article
- New Glutamine-Containing Substrates for the Assay of Cysteine Peptidases From the C1 Papain Family.Frontiers in molecular biosciences · 2020Article
- RNAiSeq: How to See the Big Picture.Frontiers in microbiology · 2019Review
- Gene expression inPeerJ · 2019Article
- RNA-Seq Validation of RNAi Identifies Additional Gene Connectivity in Tribolium castaneum (Coleoptera: Tenebrionidae).Journal of insect science (Online) · 2017Article
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The red flour beetle, Tribolium castaneum, is a major agricultural pest responsible for considerable loss of stored grain and cereal products worldwide. T. castaneum larvae have a highly compartmentalized gut, with cysteine peptidases mostly in the acidic anterior part of the midgut that are critical to the early stages of food digestion. In previous studies, we described 26 putative cysteine peptidase genes in T. castaneum (types B, L, O, F, and K) located mostly on chromosomes 3, 7, 8, and 10. In the present study, we hypothesized that specific cysteine peptidase genes could be associated with digestive functions for food processing based on comparison of gene expression profiles in different developmental stages, feeding and non-feeding. RNA-Seq was used to determine the relative expression of cysteine peptidase genes among four major developmental stages (egg, larvae, pupae, and adult) of T. castaneum. We also compared cysteine peptidase genes in T. castaneum to those in other model insects and coleopteran pests. By combining transcriptome expression, phylogenetic comparisons, response to dietary inhibitors, and other existing data, we identified key cysteine peptidases that T. castaneum larvae and adults use for food digestion, and thus new potential targets for biologically-based control products.
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Registered trials
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