Evidence map›Paper›PMID 26819828›Full record

ArticleBiomedical optics express2016

Compact plane illumination plugin device to enable light sheet fluorescence imaging of multi-cellular organisms on an inverted wide-field microscope.

Zeyi Guan, Juhyun Lee, Hao Jiang, Siyan Dong, Nelson Jen, Tzung Hsiai, Chih-Ming Ho, Peng Fei

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
6.6field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 41 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Meta-lens light-sheet fluorescence microscopy forNanophotonics (Berlin, Germany) · 2022
    Article
  6. Review
  7. Article
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  10. Fast, 3D Isotropic Imaging of Whole Mouse Brain Using Multiangle-Resolved Subvoxel SPIM.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020
    Article
  11. Article
  12. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Zeyi GuanMechanical and Aerospace Engineering, University of California, Los Angeles, Los Angeles, 90095, USA; contributed equally.
Juhyun LeeBiomedical Engineering, University of California, Los Angeles, Los Angeles, 90095, USA; contributed equally.
Hao JiangSchool of Mechanical and Engineering, Huazhong University of Science and Technology, Wuhan, 430074, China.
Siyan DongMechanical and Aerospace Engineering, University of California, Los Angeles, Los Angeles, 90095, USA.
Nelson JenBiomedical Engineering, University of California, Los Angeles, Los Angeles, 90095, USA.
Tzung HsiaiBiomedical Engineering, University of California, Los Angeles, Los Angeles, 90095, USA; School of Medicine, University of California, Los Angeles, Los Angeles, 90095, USA.
Chih-Ming HoMechanical and Aerospace Engineering, University of California, Los Angeles, Los Angeles, 90095, USA.
Peng FeiSchool of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan, 430074, China; Mechanical and Aerospace Engineering, University of California, Los Angeles, Los Angeles, 90095, USA.
University of California, Los Angeles · USHuazhong University of Science and Technology · CN

Funding

Linking Spatial Variations Shear Stress-Oxidative StressR01HL083015 · NHLBI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI HSIAI, TZUNG K · 2006 to 2015
$3.9M
Electronica Impedance to Access Metabolically Active PlaqueR01HL118650 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI HSIAI, TZUNG K · 2014 to 2022
$3.3M
Micro-Sensors to Study Electrical and Mechanical Coupling of Injured MyocardiumR01HL111437 · NHLBI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI HSIAI, TZUNG K, XU, XIAOLEI · 2012 to 2020
$3.1M
Electrical Biophysical Forces Impact Cardiac MorphogenesisR01HD069305 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHI, NEIL C · 2011 to 2015
$1.6M
NHLBI NIH HHS R01 HL083015NHLBI NIH HHS R01 HL111437NHLBI NIH HHS R01 HL118650NICHD NIH HHS R01 HD069305
6 · The paper itself

Abstract

We developed a compact plane illumination plugin (PIP) device which enabled plane illumination and light sheet fluorescence imaging on a conventional inverted microscope. The PIP device allowed the integration of microscope with tunable laser sheet profile, fast image acquisition, and 3-D scanning. The device is both compact, measuring approximately 15 by 5 by 5 cm, and cost-effective, since we employed consumer electronics and an inexpensive device molding method. We demonstrated that PIP provided significant contrast and resolution enhancement to conventional microscopy through imaging different multi-cellular fluorescent structures, including 3-D branched cells in vitro and live zebrafish embryos. Imaging with the integration of PIP greatly reduced out-of-focus contamination and generated sharper contrast in acquired 2-D plane images when compared with the stand-alone inverted microscope. As a result, the dynamic fluid domain of the beating zebrafish heart was clearly segmented and the functional monitoring of the heart was achieved. Furthermore, the enhanced axial resolution established by thin plane illumination of PIP enabled the 3-D reconstruction of the branched cellular structures, which leads to the improvement on the functionality of the wide field microscopy.

Indexed as

(110.2945) Illumination design(110.3010) Image reconstruction techniques(170.2520) Fluorescence microscopy(170.3880) Medical and biological imaging(170.6900) Three-dimensional microscopy

Identifiers

PMID26819828
PMCPMC4722903
OpenAlexW2325549975

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.