ArticleInfectious diseases2016
Rapid Point-of-Care Isothermal Amplification Assay for the Detection of Malaria without Nucleic Acid Purification.
Article in Infectious diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 43 citations in OpenAlex.
- Noninvasive malaria detection beyond blood sampling.Sensors & diagnostics · 2026Review
- Point-of-Care Extraction-Free Sample Preparation for Recombinase Polymerase Amplification of Nucleic Acid Targets in Buccal Swabs or Blood.ACS omega · 2025Article
- Point-of-care isothermal nucleic acid amplification tests: progress and bottlenecks for extraction-free sample collection and preparation.Expert review of molecular diagnostics · 2024Review
- Maximising Affordability of Real-Time Colorimetric LAMP Assays.Micromachines · 2023Article
- Evaluation of A Simple DNA Extraction Method and Its Combination with Loop-Mediated Isothermal Amplification Assays for RapidTropical medicine and infectious disease · 2023Article
- Handheld Purification-Free Nucleic Acid Testing Device for Point-of-Need Detection of Malaria from Whole Blood.ACS sensors · 2023Article
- Comparison of Reverse Transcriptase (RT) Activities of Various M-MuLV RTs for RT-LAMP Assays.Biology · 2022Article
- Urine and Saliva: Relevant Specimens for Malaria Diagnosis?Diagnostics (Basel, Switzerland) · 2022Review
- Sample-to-Answer Microfluidic Nucleic Acid Testing (NAT) on Lab-on-a-Disc for Malaria Detection at Point of Need.Methods in molecular biology (Clifton, N.J.) · 2022Article
- An Ultracompact Real-Time Fluorescence Loop-Mediated Isothermal Amplification (LAMP) Analyzer.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Towards the use of a smartphone imaging-based tool for point-of-care detection of asymptomatic low-density malaria parasitaemia.Malaria journal · 2021Article
- Development of Two-Tube Loop-Mediated Isothermal Amplification Assay for Differential Diagnosis ofDiagnostics (Basel, Switzerland) · 2021Article
- Multicopy targets for Plasmodium vivax and Plasmodium falciparum detection by colorimetric LAMP.Malaria journal · 2021Article
- Rapid visual detection of SARS-CoV-2 by colorimetric loop-mediated isothermal amplification.BioTechniques · 2021Article
- Performance and Application of Commercially Available Loop-Mediated Isothermal Amplification (LAMP) Kits in Malaria Endemic and Non-Endemic Settings.Diagnostics (Basel, Switzerland) · 2021Review
- Development of Loop-Mediated Isothermal Amplification Assay for Detection of Clinically Significant Members ofFrontiers in molecular biosciences · 2021Article
- A highly effective reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for the rapid detection of SARS-CoV-2 infection.The Journal of infection · 2021Article
- Advances in Directly Amplifying Nucleic Acids from Complex Samples.Biosensors · 2019Review
- Poor Diagnostic Performance of a Species-Specific Loop-Mediated Isothermal Amplification (LAMP) Platform for Malaria.European journal of microbiology & immunology · 2018Article
- Sample-to-answer palm-sized nucleic acid testing device towards low-cost malaria mass screening.Biosensors & bioelectronics · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Malaria remains one of the most prevalent infectious diseases and results in significant mortality. Isothermal amplification (loop-mediated isothermal amplification) is used to detect malarial DNA at levels of ~1 parasite/µL blood in ≤30 minutes without the isolation of parasite nucleic acid from subject's blood or saliva. The technique targets the mitochondrial cytochrome oxidase subunit 1 gene and is capable of distinguishing Plasmodium falciparum from Plasmodium vivax. Malarial diagnosis by the gold standard microscopic examination of blood smears is generally carried out only after moderate-to-severe symptoms appear. Rapid diagnostic antigen tests are available but generally require infection levels in the range of 200-2,000 parasites/µL for a positive diagnosis and cannot distinguish if the disease has been cleared due to the persistence of circulating antigen. This study describes a rapid and simple molecular assay to detect malarial genes directly from whole blood or saliva without DNA isolation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.