ArticleBiology of reproduction2016
A Specific Transitory Increase in Intracellular Calcium Induced by Progesterone Promotes Acrosomal Exocytosis in Mouse Sperm.
Article in Biology of reproduction, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 52 citations in OpenAlex.
- Functions of ectodysplasin A2 receptor (EDA2R) in inducing capacitation of sperm in mice.In vitro cellular & developmental biology. Animal · 2025Article
- Zika virus non-structural protein NS2A mediated endoplasmic reticulum stress through interacting with Sarco/endoplasmic reticulum CaJournal of virology · 2025Article
- Dynamic relocation of PKA regulatory subunits during sperm capacitation: Linking PKA to the CatSper signaling complex.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Real-Time Acrosome Reaction and Intracellular Calcium Recordings in Mice Sperm.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Intracellular MgFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Article
- Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization.eLife · 2024Article
- A side-by-side comparison of different capacitation media in developing mouse sperm fertilizing ability.Scientific reports · 2024Article
- Sperm calcium flux and membrane potential hyperpolarization observed in the Mexican big-eared bat Corynorhinus mexicanus.The Journal of experimental biology · 2023Article
- α-Synuclein is required for sperm exocytosis at a post-fusion stage.Frontiers in cell and developmental biology · 2023Article
- The early molecular events leading to COFILIN phosphorylation during mouse sperm capacitation are essential for acrosomal exocytosis.The Journal of biological chemistry · 2022Article
- Mysteries and unsolved problems of mammalian fertilization and related topics.Biology of reproduction · 2022Review
- Quantitative phosphoproteomics analyses reveal the regulatory mechanisms related to frozen-thawed sperm capacitation and acrosome reaction in yak (Frontiers in physiology · 2022Article
- Occurrence of Calcium Oscillations in Human Spermatozoa Is Based on Spatial Signaling Enzymes Distribution.International journal of molecular sciences · 2021Article
- Starvation induces an increase in intracellular calcium and potentiates the progesterone-induced mouse sperm acrosome reaction.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Mathematical model reveals that heterogeneity in the number of ion transporters regulates the fraction of mouse sperm capacitation.PloS one · 2021Article
- Bisphenol A Diglycidyl Ether (BADGE) and Progesterone Do Not Induce CaFrontiers in physiology · 2020Article
- Human sperm ion channel (dys)function: implications for fertilization.Human reproduction update · 2019Article
- Membrane Potential Assessment by Fluorimetry as a Predictor Tool of Human Sperm Fertilizing Capacity.Frontiers in cell and developmental biology · 2019Article
- Only a subpopulation of mouse sperm displays a rapid increase in intracellular calcium during capacitation.Journal of cellular physiology · 2018Article
- Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis.Journal of cell science · 2018Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
During capacitation, sperm acquire the ability to undergo the acrosome reaction (AR), an essential step in fertilization. Progesterone produced by cumulus cells has been associated with various physiological processes in sperm, including stimulation of AR. An increase in intracellular Ca(2+) ([Ca(2+)]i) is necessary for AR to occur. In this study, we investigated the spatiotemporal correlation between the changes in [Ca(2+)]i and AR in single mouse spermatozoa in response to progesterone. We found that progesterone stimulates an [Ca(2+)]i increase in five different patterns: gradual increase, oscillatory, late transitory, immediate transitory, and sustained. We also observed that the [Ca(2+)]i increase promoted by progesterone starts at either the flagellum or the head. We validated the use of FM4-64 as an indicator for the occurrence of the AR by simultaneously detecting its fluorescence increase and the loss of EGFP in transgenic EGFPAcr sperm. For the first time, we have simultaneously visualized the rise in [Ca(2+)]i and the process of exocytosis in response to progesterone and found that only a specific transitory increase in [Ca(2+)]i originating in the sperm head promotes the initiation of AR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.