ArticleJournal of leukocyte biology2016
Microfluidic assay for precise measurements of mouse, rat, and human neutrophil chemotaxis in whole-blood droplets.
Article in Journal of leukocyte biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 34 citations in OpenAlex.
- Leveraging Microphysiological Systems to Facilitate Neutrophil-Based Cancer Immunotherapy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Murine Neutrophil Chemotaxis Following Burn Injury with Poloxamer 188 Treatment in a Microfluidic Platform.bioRxiv : the preprint server for biology · 2026Article
- CXCR2 mediated trafficking of neutrophils and neutrophil extracellular traps are required for myelin clearance after a peripheral nerve injury.Experimental neurology · 2024Article
- Modelling the innate immune system in microphysiological systems.Lab on a chip · 2024Review
- Immuno-Kachiks formula immunomodulates and ameliorates hepatic damage induced by monosodium glutamate in rats.Heliyon · 2024Article
- Passive redirection filters minimize red blood cell contamination during neutrophil chemotaxis assays using whole blood.Lab on a chip · 2023Article
- Ex vivo observation of granulocyte activity during thrombus formation.BMC biology · 2022Article
- Review
- Electrotaxis-on-Chip to Quantify Neutrophil Migration Towards Electrochemical Gradients.Frontiers in immunology · 2021Article
- Bistable Mathematical Model of Neutrophil Migratory Patterns After LPS-Induced Epigenetic Reprogramming.Frontiers in genetics · 2021Article
- L-Arginine Modulates Neonatal Leukocyte Recruitment in a Gestational Age-Dependent Manner.Journal of clinical medicine · 2020Article
- Microfluidic devices for neutrophil chemotaxis studies.Journal of translational medicine · 2020Review
- Surface engineering within a microchannel for hydrodynamic and self-assembled cell patterning.Biomicrofluidics · 2020Article
- The Evolution of Animal Models in Wound Healing Research: 1993-2017.Advances in wound care · 2019Review
- Bifunctional Small Molecules Enhance Neutrophil Activities AgainstFrontiers in immunology · 2019Article
- Super-Low Dose Lipopolysaccharide Dysregulates Neutrophil Migratory Decision-Making.Frontiers in immunology · 2019Article
- Inflammation-on-a-Chip: Probing the Immune System Ex Vivo.Trends in biotechnology · 2018Review
- Large-scale patterning of living colloids for dynamic studies of neutrophil-microbe interactions.Lab on a chip · 2018Article
- Neutrophils from Both Susceptible and Resistant Mice Efficiently Kill Opsonized Listeria monocytogenes.Infection and immunity · 2018Article
- MBiosensors & bioelectronics · 2018Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
Animal models of human disease differ in innate immune responses to stress, pathogens, or injury. Precise neutrophil phenotype measurements could facilitate interspecies comparisons. However, such phenotype comparisons could not be performed accurately with the use of current assays, as they require the separation of neutrophils from blood using species-specific protocols, and they introduce distinct artifacts. Here, we report a microfluidic technology that enables robust characterization of neutrophil migratory phenotypes in a manner independent of the donor species and performed directly in a droplet of whole blood. The assay relies on the particular ability of neutrophils to deform actively during chemotaxis through microscale channels that block the advance of other blood cells. Neutrophil migration is measured directly in blood, in the presence of other blood cells and serum factors. Our measurements reveal important differences among migration counts, velocity, and directionality among neutrophils from 2 common mouse strains, rats, and humans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.