ArticleScientific reports2016
Reference genes for accessing differential expression among developmental stages and analysis of differential expression of OBP genes in Anastrepha obliqua.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
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- Young Goji Fruit Volatiles Regulate the Oviposition Behavior and Chemosensory Gene Expression of Gravid FemaleInternational journal of molecular sciences · 2024Article
- Selection and Validation of Reference Genes for Gene Expression inInternational journal of molecular sciences · 2024Article
- Nursing Honeybee Behavior and Sensorial-Related Genes Are Altered by Deformed Wing Virus Variant A.Insects · 2024Article
- Transcriptome-Based Selection and Validation of Reference Genes for Gene Expression in Goji Fruit Fly (International journal of molecular sciences · 2022Article
- Reference Genes for Expression Analysis Using RT-qPCR inInsects · 2022Article
- Selection of species specific panel of reference genes in peripheral blood mononuclear cells of native livestock species adapted to trans-Himalayan region of Leh-Ladakh.Scientific reports · 2022Article
- Identification of Candidate Chemosensory Gene Families by Head Transcriptomes Analysis in the Mexican Fruit Fly,International journal of molecular sciences · 2022Article
- Reference Genes for Expression Analyses by qRT-PCR inInsects · 2022Article
- Selection and Validation of Reference Genes for Quantitative Real-Time PCR Normalization inFrontiers in physiology · 2022Article
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- Identification and Evaluation of Suitable Reference Genes for RT-qPCR Analysis inFrontiers in physiology · 2021Article
- Transcriptome analysis of Anastrepha fraterculus sp. 1 males, females, and embryos: insights into development, courtship, and reproduction.BMC genetics · 2020Article
- A First Glimpse of the Mexican Fruit FlyInternational journal of molecular sciences · 2020Article
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- Protein Stores Regulate When Reproductive Displays Begin in the Male Caribbean Fruit Fly.Frontiers in physiology · 2020Article
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Authors and funding
7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The West Indian fruit fly, Anastrepha obliqua, is an important agricultural pest in the New World. The use of pesticide-free methods to control invasive species such as this reinforces the search for genes potentially useful in their genetic control. Therefore, the study of chemosensory proteins involved with a range of responses to the chemical environment will help not only on the understanding of the species biology but may also help the development of environmentally friendly pest control strategies. Here we analyzed the expression patterns of three OBP genes, Obp19d_2, Obp56a and Obp99c, across different phases of A. obliqua development by qPCR. In order to do so, we tested eight and identified three reference genes for data normalization, rpl17, rpl18 and ef1a, which displayed stability for the conditions here tested. All OBPs showed differential expression on adults and some differential expression among adult stages. Obp99c had an almost exclusive expression in males and Obp56a showed high expression in virgin females. Thereby, our results provide relevant data not only for other gene expression studies in this species, as well as for the search of candidate genes that may help in the development of new pest control strategies.
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