ArticleJournal of clinical microbiology2016
Detecting Staphylococcus aureus Virulence and Resistance Genes: a Comparison of Whole-Genome Sequencing and DNA Microarray Technology.
Article in Journal of clinical microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 51 citations in OpenAlex.
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- Case Report: Fatal Necrotizing Pneumonia by Exfoliative ToxinMicroorganisms · 2025Article
- Rapid Detection of Panton-Valentine Leukocidin Production in Clinical Isolates ofPathogens (Basel, Switzerland) · 2025Article
- Detecting antibiotic resistance: classical, molecular, advanced bioengineering, and AI-enhanced approaches.Frontiers in microbiology · 2025Review
- Genetic Characterization of Antibiotic-ResistantAntibiotics (Basel, Switzerland) · 2024Article
- Whole genome sequencing and molecular epidemiology of methicillin-resistant Staphylococcus aureus isolated from patients with bacteraemia in Slovenia.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- Genetic Determinants in MRSA Carriage and Their Association with Decolonization Outcome.Current microbiology · 2024Article
- Panton-Valentine Leukocidin-Positive CC398 MRSA in Urban Clinical Settings, the Netherlands.Emerging infectious diseases · 2023Article
- Prospective surveillance of colonization and disease by methicillin-resistant Staphylococcus aureus (MRSA) at a European pediatric cancer center.Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer · 2022Observational
- Importance ofThe Journal of international medical research · 2022Article
- Virulence Determinants inFrontiers in microbiology · 2022Article
- Epidemiology of the Staphylococcus aureus CA-MRSA USA300 in Belgium.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Article
- Molecular Characterization ofPathogens (Basel, Switzerland) · 2021Article
- Genomic analysis of Staphylococcus aureus from the West African Dwarf (WAD) goat in Nigeria.Antimicrobial resistance and infection control · 2021Article
- Harmonization of whole-genome sequencing for outbreak surveillance ofMicrobial genomics · 2021Article
- Molecular investigations on a chimeric strain of Staphylococcus aureus sequence type 80.PloS one · 2020Article
- Origin and Evolution of Hybrid Shiga Toxin-Producing and Uropathogenic Escherichia coli Strains of Sequence Type 141.Journal of clinical microbiology · 2019Article
- Article
- Integrating Culture-based Antibiotic Resistance Profiles with Whole-genome Sequencing Data for 11,087 Clinical Isolates.Genomics, proteomics & bioinformatics · 2019Article
- Coagulase-Negative Staphylococci Pathogenomics.International journal of molecular sciences · 2019Review
Corrections and comments
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Authors and funding
17 authors at 8 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Staphylococcus aureusis a major bacterial pathogen causing a variety of diseases ranging from wound infections to severe bacteremia or intoxications. Besides host factors, the course and severity of disease is also widely dependent on the genotype of the bacterium. Whole-genome sequencing (WGS), followed by bioinformatic sequence analysis, is currently the most extensive genotyping method available. To identify clinically relevant staphylococcal virulence and resistance genes in WGS data, we developed anin silicotyping scheme for the software SeqSphere(+)(Ridom GmbH, Münster, Germany). The implemented target genes (n= 182) correspond to those queried by the IdentibacS. aureusGenotyping DNA microarray (Alere Technologies, Jena, Germany). Thein silicoscheme was evaluated by comparing the typing results of microarray and of WGS for 154 humanS. aureusisolates. A total of 96.8% (n= 27,119) of all typing results were equally identified with microarray and WGS (40.6% present and 56.2% absent). Discrepancies (3.2% in total) were caused by WGS errors (1.7%), microarray hybridization failures (1.3%), wrong prediction of ambiguous microarray results (0.1%), or unknown causes (0.1%). Superior to the microarray, WGS enabled the distinction of allelic variants, which may be essential for the prediction of bacterial virulence and resistance phenotypes. Multilocus sequence typing clonal complexes and staphylococcal cassette chromosomemecelement types inferred from microarray hybridization patterns were equally determined by WGS. In conclusion, WGS may substitute array-based methods due to its universal methodology, open and expandable nature, and rapid parallel analysis capacity for different characteristics in once-generated sequences.
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