ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2016
Surveillance of a Ventilated Rack System for Corynebacterium bovis by Sampling Exhaust-Air Manifolds.
Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- Pooled it
- Prevalence of Large Academic Research Institutions and NCI Designated Cancer Centers with Corynebacterium bovis Infections among Immunodeficient Mice in the United States from 2015 to 2023.Journal of the American Association for Laboratory Animal Science : JAALAS · 2026Article
- Evaluation of Direct Colony Sampling Compared with Sentinel-Free Soiled Bedding Testing for Murine Quarantine Programs.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- Detection and Remediation of Pneumocystis murina Infections by Environmental Health Monitoring.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- A survey on the implementation of environmental health monitoring in mouse facilities in German-speaking countries.PloS one · 2025Article
- A Clinical Scoring System and Impact of Athymic Nude Mouse Age onComparative medicine · 2024Article
- Comparison of Rodent Infectious Agent Detection by Exhaust Dust Testing and Traditional Sentinel Testing Using Quantitative Polymerase Chain Reaction.Journal of the American Association for Laboratory Animal Science : JAALAS · 2024Article
- Establishing the Median Infectious Dose and Characterizing the Clinical Manifestations of Mouse, Rat, Cow, and HumanComparative medicine · 2023Article
- A Cross-sectional Survey on Rodent Environmental Health Monitoring Practices: Benchmarking, Associations, and Barriers.Journal of the American Association for Laboratory Animal Science : JAALAS · 2023Article
- Microbiota and environmental health monitoring of mouse colonies by metagenomic shotgun sequencing.World journal of microbiology & biotechnology · 2022Article
- A Clinical Scoring Systems for the Evaluation ofComparative medicine · 2022Article
- Single-tube Multiplex Nested PCR System for Efficient Detection of Pathogenic Microorganisms in SPF Rodents.Journal of the American Association for Laboratory Animal Science : JAALAS · 2022Article
- Effect of Antimicrobial Prophylaxis onComparative medicine · 2022Article
- Antimicrobial Susceptibility ofComparative medicine · 2021Article
- PCR and RT-PCR in the Diagnosis of Laboratory Animal Infections and in Health Monitoring.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- Searching for a Bacteriophage Lysin to TreatComparative medicine · 2020Article
- Detection of Lactate Dehydrogenase Elevating Virus in a Mouse Vivarium Using an Exhaust Air Dust Health Monitoring Program.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- Effects ofComparative medicine · 2019Article
- PCR Prevalence of Murine Opportunistic Microbes and their Mitigation by Using Vaporized Hydrogen Peroxide.Journal of the American Association for Laboratory Animal Science : JAALAS · 2019Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Corynebacterium bovis causes an opportunistic infection of nude (Foxn1, nu/nu) mice, leading to nude mouse hyperkeratotic dermatitis (scaly skin disease). Enzootic in many nude mouse colonies, C. bovis spreads rapidly to naive nude mice, despite modern husbandry practices, and is very difficult to eradicate. To facilitate rapid detection in support of eradication efforts, we investigated a surveillance method based on quantitative real-time PCR (qPCR) evaluation of swabs collected from the horizontal exhaust manifold (HEM) of an IVC rack system. We first evaluated the efficacy of rack sanitation methods for removing C. bovis DNA from the HEM of racks housing endemic colonies of infected nude mice. Pressurized water used to flush the racks' air exhaust system followed by a standard rack-washer cycle was ineffective in eliminating C. bovis DNA. Only after autoclaving did all sanitized racks test negative for C. bovis DNA. We then measured the effects of stage of infection (early or established), cage density, and cage location on the rack on time-to-detection at the HEM. Stage of infection significantly affected time-to-detection, independent of cage location. Early infections required 7.3 ± 1.2 d whereas established infections required 1 ± 0 d for detection of C. bovis at the HEM. Cage density influenced the quantity of C. bovis DNA detected but not time-to-detection. The location of the cage on the rack affected the time-to-detection only during early C. bovis infections. We suggest that qPCR swabs of HEM are useful during the routine surveillance of nude mouse colonies for C. bovis infection.
Indexed as
Identifiers
26817981PMC4747012W2444160974What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.