ArticleThe Journal of biological chemistry2016
Identification of pH-sensing Sites in the Light Harvesting Complex Stress-related 3 Protein Essential for Triggering Non-photochemical Quenching in Chlamydomonas reinhardtii.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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66 citing papers in PubMed, 118 citations in OpenAlex.
- From proton microdomains to protein migration: hypothesis for ΔpH-controlled PSII relocation.Photosynthesis research · 2026Article
- Acclimation to high and low diurnal light is flexible inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Mechanisms of light harvesting complex proteins in photoprotection of the brown tide alga.Nature communications · 2025Article
- Too dim, too bright, and just right: Systems analysis of the Chlamydomonas diurnal program under limiting and excess light.The Plant cell · 2025Article
- Macroscale structural changes of thylakoid architecture during high light acclimation in Chlamydomonas reinhardtii.Photosynthesis research · 2024Article
- Fluorescence and electron transfer of Limnospira indica functionalized biophotoelectrodes.Photosynthesis research · 2024Article
- Regulation of Microalgal Photosynthetic Electron Transfer.Plants (Basel, Switzerland) · 2024Review
- Chemical Protein Crosslinking-Coupled Mass Spectrometry Reveals Interaction of LHCI with LHCII and LHCSR3 inPlants (Basel, Switzerland) · 2024Article
- Proteomic characterization of a lutein-hyperaccumulating Chlamydomonas reinhardtii mutant reveals photoprotection-related factors as targets for increasing cellular carotenoid content.Biotechnology for biofuels and bioproducts · 2023Article
- Characterizing compensatory mechanisms in the absence of photoprotective qE in Chlamydomonas reinhardtii.Photosynthesis research · 2023Article
- Weak acids produced during anaerobic respiration suppress both photosynthesis and aerobic respiration.Nature communications · 2023Article
- How the pH Controls Photoprotection in the Light-Harvesting Complex of Mosses.Journal of the American Chemical Society · 2023Article
- LHC-like Proteins: The Guardians of Photosynthesis.International journal of molecular sciences · 2023Review
- Article
- The relationship between photosystem II regulation and light-dependent hydrogen production by microalgae.Biophysical reviews · 2022Review
- Macromolecular conformational changes in photosystem II: interaction between structure and function.Biophysical reviews · 2022Review
- Interplay between LHCSR proteins and state transitions governs the NPQ response in Chlamydomonas during light fluctuations.Plant, cell & environment · 2022Article
- Engineering astaxanthin accumulation reduces photoinhibition and increases biomass productivity under high light in Chlamydomonas reinhardtii.Biotechnology for biofuels and bioproducts · 2022Article
- Transcriptional regulation of photoprotection in dark-to-light transition-More than just a matter of excess light energy.Science advances · 2022Article
- The role of photosynthesis related pigments in light harvesting, photoprotection and enhancement of photosynthetic yield in planta.Photosynthesis research · 2022Review
6 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Light harvesting complex stress-related 3 (LHCSR3) is the protein essential for photoprotective excess energy dissipation (non-photochemical quenching, NPQ) in the model green algaChlamydomonas reinhardtii Activation of NPQ requires low pH in the thylakoid lumen, which is induced in excess light conditions and sensed by lumen-exposed acidic residues. In this work we have used site-specific mutagenesisin vivoandin vitrofor identification of the residues in LHCSR3 that are responsible for sensing lumen pH. Lumen-exposed protonatable residues, aspartate and glutamate, were mutated to asparagine and glutamine, respectively. By expression in a mutant lacking all LHCSR isoforms, residues Asp(117), Glu(221), and Glu(224)were shown to be essential for LHCSR3-dependent NPQ induction inC. reinhardtii Analysis of recombinant proteins carrying the same mutations refoldedin vitrowith pigments showed that the capacity of responding to low pH by decreasing the fluorescence lifetime, present in the wild-type protein, was lost. Consistent with a role in pH sensing, the mutations led to a substantial reduction in binding the NPQ inhibitor dicyclohexylcarbodiimide.
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