ArticleJournal of vascular surgery2017
Eph-B4 mediates vein graft adaptation by regulation of endothelial nitric oxide synthase.
Article in Journal of vascular surgery, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Sex differences in arterial identity correlate with neointimal hyperplasia after balloon injury.Molecular biology reports · 2022Article
- Sex Differences in Inflammation During Venous Remodeling of Arteriovenous Fistulae.Frontiers in cardiovascular medicine · 2021Review
- The Pyrazolo[3,4-Molecules (Basel, Switzerland) · 2020Article
- Molecular identity of arteries, veins, and lymphatics.Journal of vascular surgery · 2019Review
- Arterial Venous Differentiation for Vascular Bioengineering.Annual review of biomedical engineering · 2018Review
- Dual Function for Mature Vascular Smooth Muscle Cells During Arteriovenous Fistula Remodeling.Journal of the American Heart Association · 2017Article
- Improving the Outcome of Vein Grafts: Should Vascular Surgeons Turn Veins into Arteries?Annals of vascular diseases · 2017Review
- Membrane-mediated regulation of vascular identity.Birth defects research. Part C, Embryo today : reviews · 2016Review
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8 authors.
Funding
Abstract
objectiveVein graft adaptation is characterized by loss of expression of the tyrosine kinase receptor Eph-B4, the embryonic determinant of venous identity, without increased expression of its ligand ephrin-B2, the embryonic determinant of arterial identity. Endothelial nitric oxide synthase (eNOS) is an important mediator of vessel remodeling. We hypothesized that the mechanism of action of Eph-B4 during vein graft adaptation might be through regulation of downstream eNOS activity.
methodsMouse lung endothelial cells were stimulated with ephrin-B2/Fc, without and with preclustering, without and with the eNOS inhibitor N
resultsEphrin-B2/Fc stimulated both Eph-B4 and eNOS phosphorylation in a bimodal temporal distribution (n = 4; P < .05), with preclustered ephrin-B2/Fc causing prolonged peak Eph-B4 and eNOS phosphorylation as well as altered subcellular localization (n = 4; P < .05). Ephrin-B2/Fc increased NO release (n = 3; P < .01) as well as increased endothelial cell migration (n = 6; P < .05) in an eNOS-dependent fashion. Both human and mouse vein grafts showed increased eNOS phosphorylation compared with normal veins (n = 3; P < .05). Vein grafts from eNOS knockout mice showed less dilation and less wall thickening compared with wild-type vein grafts (n = 7; P < .05).
conclusionseNOS is a mediator of vein graft adaptation to the arterial environment. Eph-B4 stimulates eNOS phosphorylation in vitro and may mediate vein graft adaptation by regulation of eNOS activity in vivo.
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