Evidence map›Paper›PMID 26816587›Full record

ArticleElectronic physician2015

Accuracy of universal polymerase chain reaction (PCR) for detection of bacterial meningitis among suspected patients.

Ali Reza Moayedi, Abdolazim Nejatizadeh, Maryam Mohammadian, Mohammad Bagher Rahmati, Vahideh Namardizadeh

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Electronic physician, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Laboratory Diagnosis of Central Nervous System Infection.Current infectious disease reports · 2016
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Ali Reza MoayediM.D., Assistant Professor, Department of Pediatric Neurology, Faculty of Medicine, Clinical Research Development Center of Children Hospital, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Abdolazim NejatizadehPh.D. of Genetics, Associate Professor, Department of Genetics, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Maryam MohammadianM.D., Pediatric Resident, Department of Pediatrics, Faculty of Medicine, Clinical Research Development Center of Children Hospital, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Mohammad Bagher RahmatiM.D., Assistant Professor, Department of Pediatric Infectious Diseases, Faculty of Medicine, Infectious and tropical diseases research center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Vahideh NamardizadehB.Sc., Department of Genetics, Faculty of Medicine, Khalije-Fars Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Hormozgan University of Medical Sciences · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionCentral nervous system (CNS) infections are life-threatening diseases caused by viral, bacterial, parasitic and fungal microorganisms. The aim of this study was to determine the accuracy of universal polymerase chain reaction (PCR) for the detection of bacterial meningitis among patients who were referred to Koodakan Hospital in Bandar Abbas because they were suspected of having the disease.

methodsThis study was conducted in 2013 on the patients who were admitted to Bandar Abbas' Koodakan Hospital because they were suspected of having meningitis. A questionnaire, including demographic data, was completed for each patient. Universal PCR, Cerebrospinal fluid (CSF) analysis, and gram staining and cultures were done for all the patients. The data were analyzed using SPSS software.

resultsAmong the 100 patients studied 59 (59%) were male and 41 (41%) were female. No patient in our study had a positive smear and culture for meningitis. Among the patients with negative smears and cultures six (6%) had positive universal PCR, and 94 (94%) had negative universal PCR. Based on these results, PCR had 95% specificity and 100% negative predictive value for the prediction of meningitis. In 30 patients (30%), the biochemical analysis of CSF were in favor of meningitis. Among the 30 patients, six patients (20%) had positive universal PCR and 24 patients (80%) had negative universal PCR.

conclusionBased on our results, the universal PCR test is useful in the diagnosis of bacterial meningitis in children. We recommend using it in combination with other tests, such as CSF analysis, for diagnosis of bacterial meningitis.

Indexed as

gram stain and culturemeningitisuniversal PCR

Identifiers

PMID26816587
PMCPMC4725414
OpenAlexW2283367209

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.