ArticleAnalytical chemistry2016
hνSABR: Photochemical Dose-Response Bead Screening in Droplets.
Article in Analytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Automated DNA-Encoded Library Synthesis and Activity-Based Screening at the Attomole Scale.Journal of medicinal chemistry · 2026Article
- Solid-phase DNA-encoded library synthesis: a master builder's instructions.Nature protocols · 2026Review
- Droplet-Based Microfluidics Methods for Detecting Enzyme Inhibitors.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A Functional Assay for Mining Noninhibitory Enzyme Ligands from One Bead One Compound Libraries: Application to E3 Ubiquitin Ligases.Journal of the American Chemical Society · 2025Article
- My Perspective on OBOC Combinatorial Technology.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Dose-Response Activity-Based DNA-Encoded Library Screening.ACS medicinal chemistry letters · 2023Article
- Liposomal Permeation Assay for Droplet-Scale Pharmacokinetic Screening.Journal of medicinal chemistry · 2023Article
- Translating the Genome into Drugs.Accounts of chemical research · 2023Article
- Chiral lipid bilayers are enantioselectively permeable.Nature chemistry · 2021Article
- Active Flow Control and Dynamic Analysis in Droplet Microfluidics.Annual review of analytical chemistry (Palo Alto, Calif.) · 2021Review
- DNA-Encoded Chemistry: Drug Discovery from a Few Good Reactions.Chemical reviews · 2021Review
- Considerations for Achieving Maximized DNA Recovery in Solid-Phase DNA-Encoded Library Synthesis.ACS combinatorial science · 2020Article
- A microarray platform designed for high-throughput screening the reaction conditions for the synthesis of micro/nanosized biomedical materials.Bioactive materials · 2020Article
- Off-DNA DNA-Encoded Library Affinity Screening.ACS combinatorial science · 2020Article
- Beyond protein binding: recent advances in screening DNA-encoded libraries.Chemical communications (Cambridge, England) · 2019Review
- Developments with bead-based screening for novel drug discovery.Expert opinion on drug discovery · 2019Article
- Activity-Based DNA-Encoded Library Screening.ACS combinatorial science · 2019Article
- Acoustic Droplet Ejection Enabled Automated Reaction Scouting.ACS central science · 2019Article
- Droplet Microfluidics in Thermoplastics: Device Fabrication, Droplet Generation, and Content Manipulation using Integrated Electric and Magnetic Fields.Analytical methods : advancing methods and applications · 2018Article
- Detection of antibiotics synthetized in microfluidic picolitre-droplets by various actinobacteria.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
With the potential for each droplet to act as a unique reaction vessel, droplet microfluidics is a powerful tool for high-throughput discovery. Any attempt at compound screening miniaturization must address the significant scaling inefficiencies associated with library handling and distribution. Eschewing microplate-based compound collections for one-bead-one-compound (OBOC) combinatorial libraries, we have developed hνSABR (Light-Induced and -Graduated High-Throughput Screening After Bead Release), a microfluidic architecture that integrates a suspension hopper for compound library bead introduction, droplet generation, microfabricated waveguides to deliver UV light to the droplet flow for photochemical compound dosing, incubation, and laser-induced fluorescence for assay readout. Avobenzone-doped PDMS (0.6% w/w) patterning confines UV exposure to the desired illumination region, generating intradroplet compound concentrations (>10 μM) that are reproducible between devices. Beads displaying photochemically cleavable pepstatin A were distributed into droplets and exposed with five different UV intensities to demonstrate dose-response screening in an HIV-1 protease activity assay. This microfluidic architecture introduces a new analytical approach for OBOC library screening, and represents a key component of a next-generation distributed small molecule discovery platform.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.