ArticleNature communications2016
Electrochemical camera chip for simultaneous imaging of multiple metabolites in biofilms.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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Who cites it
46 citing papers in PubMed, 120 citations in OpenAlex.
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- Microbial phenazines: biosynthesis, structural diversity, evolution, regulation, and biological significance.Microbiology and molecular biology reviews : MMBR · 2025Review
- Single-cell lysis patterns morphogenesis of eDNA in the matrix ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Use of analytical strategies to understand spatial chemical variation in bacterial surface communities.Journal of bacteriology · 2025Review
- Quantification of Pseudomonas aeruginosa biofilms using electrochemical methods.Access microbiology · 2025Article
- Real-time high-resolution microscopy reveals how single-cell lysis shapes biofilm matrix morphogenesis.bioRxiv : the preprint server for biology · 2024Article
- Cellular arrangement impacts metabolic activity and antibiotic tolerance in Pseudomonas aeruginosa biofilms.PLoS biology · 2024Article
- Spatial heterogeneity in biofilm metabolism elicited by local control of phenazine methylation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Rapid fingerprinting of bacterial species using nanocavities created on screen-printed electrodes modified by β-cyclodextrin.Sensors & diagnostics · 2023Article
- Cell arrangement impacts metabolic activity and antibiotic tolerance inbioRxiv : the preprint server for biology · 2023Article
- A phenazine-inspired framework for identifying biological functions of microbial redox-active metabolites.Current opinion in chemical biology · 2023Review
- NADH dehydrogenases are the predominant phenazine reductases in the electron transport chain of Pseudomonas aeruginosa.Molecular microbiology · 2023Article
- Living electronics: A catalogue of engineered living electronic components.Microbial biotechnology · 2023Review
- Cellulose-Based Laser-Induced Graphene Devices for Electrochemical Monitoring of Bacterial Phenazine Production and Viability.Sensors and actuators. B, Chemical · 2023Article
- Advances in linking single-cell bacterial stress response to population-level survival.Current opinion in biotechnology · 2023Review
- Marine biofilms: diversity, interactions and biofouling.Nature reviews. Microbiology · 2022Review
- Gradients and consequences of heterogeneity in biofilms.Nature reviews. Microbiology · 2022Review
- Electrochemical Sensors Based on MoSAdvanced healthcare materials · 2022Article
- Development of a Versatile, Low-Cost Electrochemical System to Study Biofilm Redox Activity at the Micron Scale.Applied and environmental microbiology · 2022Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Monitoring spatial distribution of metabolites in multicellular structures can enhance understanding of the biochemical processes and regulation involved in cellular community development. Here we report on an electrochemical camera chip capable of simultaneous spatial imaging of multiple redox-active phenazine metabolites produced by Pseudomonas aeruginosa PA14 colony biofilms. The chip features an 8 mm × 8 mm array of 1,824 electrodes multiplexed to 38 parallel output channels. Using this chip, we demonstrate potential-sweep-based electrochemical imaging of whole-biofilms at measurement rates in excess of 0.2 s per electrode. Analysis of mutants with various capacities for phenazine production reveals distribution of phenazine-1-carboxylic acid (PCA) throughout the colony, with 5-methylphenazine-1-carboxylic acid (5-MCA) and pyocyanin (PYO) localized to the colony edge. Anaerobic growth on nitrate confirms the O2-dependence of PYO production and indicates an effect of O2 availability on 5-MCA synthesis. This integrated-circuit-based technique promises wide applicability in detecting redox-active species from diverse biological samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.