ArticleThe Plant cell2016
Interactions of C4 Subtype Metabolic Activities and Transport in Maize Are Revealed through the Characterization of DCT2 Mutants.
Article in The Plant cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
- Exploring the diversity of the CO2-concentrating mechanism in different C4 subtypes.Journal of experimental botany · 2026Article
- The metabolite transporters of C4 photosynthesis.The Plant cell · 2025Review
- Metabolite profiling reveals slow and uncoordinated adjustment of C4 photosynthesis to sudden changes in irradiance.Plant physiology · 2025Article
- Systems analysis of long-term heat stress responses in the C4 grass Setaria viridis.The Plant cell · 2025Article
- A dominant role of transcriptional regulation during the evolution of CNature communications · 2025Article
- Genomic and Transcriptomic Insights into the Evolution of C4 Photosynthesis in Grasses.Genome biology and evolution · 2024Article
- SIMPEL: using stable isotopes to elucidate dynamics of context specific metabolism.Communications biology · 2024Article
- Review
- 13CO2 labeling kinetics in maize reveal impaired efficiency of C4 photosynthesis under low irradiance.Plant physiology · 2022Article
- Accelerated remodeling of the mesophyll-bundle sheath interface in the maize C4 cycle mutant leaves.Scientific reports · 2022Article
- Metabolic profiles in C3, C3-C4 intermediate, C4-like, and C4 species in the genus Flaveria.Journal of experimental botany · 2022Article
- Distinct CPlant direct · 2021Article
- Improving C4 photosynthesis to increase productivity under optimal and suboptimal conditions.Journal of experimental botany · 2021Article
- The utility of metabolomics as a tool to inform maize biology.Plant communications · 2021Review
- Transport Proteins Enabling Plant Photorespiratory Metabolism.Plants (Basel, Switzerland) · 2021Review
- DCT4-A New Member of the Dicarboxylate Transporter Family in C4 Grasses.Genome biology and evolution · 2021Article
- Plant Single-Cell Metabolomics-Challenges and Perspectives.International journal of molecular sciences · 2020Review
- Sterile Spikelets Contribute to Yield in Sorghum and Related Grasses.The Plant cell · 2020Article
- Tracing metabolic flux through time and space with isotope labeling experiments.Current opinion in biotechnology · 2020Review
- Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
C4 photosynthesis in grasses requires the coordinated movement of metabolites through two specialized leaf cell types, mesophyll (M) and bundle sheath (BS), to concentrate CO2 around Rubisco. Despite the importance of transporters in this process, few have been identified or rigorously characterized. In maize (Zea mays), DCT2 has been proposed to function as a plastid-localized malate transporter and is preferentially expressed in BS cells. Here, we characterized the role of DCT2 in maize leaves using Activator-tagged mutant alleles. Our results indicate that DCT2 enables the transport of malate into the BS chloroplast. Isotopic labeling experiments show that the loss of DCT2 results in markedly different metabolic network operation and dramatically reduced biomass production. In the absence of a functioning malate shuttle, dct2 lines survive through the enhanced use of the phosphoenolpyruvate carboxykinase carbon shuttle pathway that in wild-type maize accounts for ∼ 25% of the photosynthetic activity. The results emphasize the importance of malate transport during C4 photosynthesis, define the role of a primary malate transporter in BS cells, and support a model for carbon exchange between BS and M cells in maize.
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