ArticleScientific reports2016
Selection of suitable reference genes for qRT-PCR normalization during leaf development and hormonal stimuli in tea plant (Camellia sinensis).
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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69 citing papers in PubMed, 134 citations in OpenAlex.
- Selection and Validation of Suitable Reference Genes for RT-qPCR Normalization inPlants (Basel, Switzerland) · 2026Article
- Genome-Wide Analysis of the Cinnamoyl-CoA Reductase (CCR) Gene Family and Its Involvement in Lignin Biosynthesis and Stress Responses in Six Tea Plant Cultivars.International journal of molecular sciences · 2026Article
- Selection of reliable reference genes in Colletotrichum scovillei during different growth stages, host interactions, and plant extract treatment for qRT-PCR.BMC plant biology · 2025Article
- Identification and validation of reference genes for qPCR of Pseudomonas aeruginosa L10 under varying n-hexadecane concentrations.BMC microbiology · 2025Article
- Multi-omics analysis of the mechanisms of abundant theacrine and EGCG3"Me in tea (Camellia sinensis).BMC plant biology · 2025Article
- Selection and validation of reference genes in alfalfa based on transcriptome sequence data.Scientific reports · 2025Article
- Involvement of endogenous IAA and ABA in the regulation of arbuscular mycorrhizal fungus on rooting of tea plant (Camellia sinensis L.) cuttings.BMC plant biology · 2024Article
- Overexpressing CsPSY1 Gene of Tea Plant, Encoding a Phytoene Synthase, Improves α-Carotene and β-Carotene Contents in Carrot.Molecular biotechnology · 2024Article
- Interference of skeleton photoperiod in circadian clock and photosynthetic efficiency of tea plant: in-depth analysis of mathematical model.Horticulture research · 2024Article
- RNA-seq validation: software for selection of reference and variable candidate genes for RT-qPCR.BMC genomics · 2024Article
- Circadian rhythm response and its effect on photosynthetic characteristics of the Lhcb family genes in tea plant.BMC plant biology · 2024Article
- Identification and Characterisation of the CircRNAs Involved in the Regulation of Leaf Colour inBiology · 2024Article
- Article
- Differential Response of MYB Transcription Factor Gene Transcripts to Circadian Rhythm in Tea Plants (International journal of molecular sciences · 2024Article
- Article
- Exogenous methyl jasmonate and cytokinin antagonistically regulate lignin biosynthesis by mediating CsHCT expression in Camellia sinensis.Protoplasma · 2023Article
- Transcriptome Analysis Reveals Differentially Expressed Genes Involved in Cadmium and Arsenic Accumulation in Tea Plant (Plants (Basel, Switzerland) · 2023Article
- Article
- Isolation and Characterization of an LBD Transcription Factor CsLBD39 from Tea Plant (International journal of molecular sciences · 2022Article
- Exogenous Melatonin Enhances Photosynthetic Capacity and Related Gene Expression in A Dose-Dependent Manner in the Tea Plant (International journal of molecular sciences · 2022Article
9 more citing papers are in PubMed but not listed here.
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Tea plant (Camellia sinensis) leaf is an important non-alcoholic beverage resource. The application of quantitative real time polymerase chain reaction (qRT-PCR) has a profound significance for the gene expression studies of tea plant, especially when applied to tea leaf development and metabolism. In this study, nine candidate reference genes (i.e., CsACT7, CsEF-1α, CseIF-4α, CsGAPDH, CsPP2A, CsSAND, CsTBP, CsTIP41, and CsTUB) of C. sinensis were cloned. The quantitative expression data of these genes were investigated in five tea leaf developmental stages (i.e., 1st, 2nd, 3rd, 4th, and older leaves) and normal growth tea leaves subjected to five hormonal stimuli (i.e., ABA, GA, IAA, MeJA, and SA), and gene expression stability was calculated using three common statistical algorithms, namely, geNorm, NormFinder, and Bestkeeper. Results indicated that CsTBP and CsTIP41 were the most stable genes in tea leaf development and CsTBP was the best gene under hormonal stimuli; by contrast, CsGAPDH and CsTUB genes showed the least stability. The gene expression profile of CsNAM gene was analyzed to confirm the validity of the reference genes in this study. Our data provide basis for the selection of reference genes for future biological research in the leaf development and hormonal stimuli of C. sinensis.
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