ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2016
Mosaic analysis of cell rearrangements during ureteric bud branching in dissociated/reaggregated kidney cultures and in vivo.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Developmentally inspired synthetic kidney engineering.Nature biotechnology · 2026Review
- Measurement of adhesion and traction of cells at high yield reveals an energetic ratchet operating during nephron condensation.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Measurement of adhesion and traction of cells at high yield (MATCHY) reveals an energetic ratchet driving nephron condensation.bioRxiv : the preprint server for biology · 2024Article
- Rho/ROCK activity tunes cell compartment segregation and differentiation in nephron-forming niches.bioRxiv : the preprint server for biology · 2023Article
- New developments in the biology of fibroblast growth factors.WIREs mechanisms of disease · 2022Review
- Ret signaling in ureteric bud epithelial cells controls cell movements, cell clustering and bud formation.Development (Cambridge, England) · 2021Article
- Techniques of fragile renal organoids transplantation in mice.Acta cirurgica brasileira · 2021Article
- Organ-Specific Branching Morphogenesis.Frontiers in cell and developmental biology · 2021Review
- Genetic manipulation of ureteric bud tip progenitors in the mammalian kidney through an Adamts18 enhancer driven tet-on inducible system.Developmental biology · 2020Article
- Exosomes as secondary inductive signals involved in kidney organogenesis.Journal of extracellular vesicles · 2018Article
- p63+ ureteric bud tip cells are progenitors of intercalated cells.JCI insight · 2017Article
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2 authors at 1 institution in 1 country.
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Abstract
backgroundCell rearrangements mediated by GDNF/Ret signaling underlie the formation of the ureteric bud (UB) tip domain during kidney development. Whether FGF signaling also influences these rearrangements is unknown. Chimeric embryos are a powerful tool for examining the genetic controls of cellular behaviors, but generating chimeras by traditional methods is expensive and laborious. Dissociated fetal kidney cells can reorganize to form complex structures including branching UB tubules, providing an easier method to generate renal chimeras.
resultsCell behaviors in normal or chimeric kidney cultures were investigated using time-lapse imaging. In Spry1(-/-) ↔ wild-type chimeras, cells lacking Spry1 (a negative regulator of Ret and FGF receptor signaling) preferentially occupied the UB tips, as previously observed in traditional chimeras, thus validating this experimental system. In Fgfr2(UB-/-) ↔ wild-type chimeras, the wild-type cells preferentially occupied the tips. Independent evidence for a role of Fgfr2 in UB tip formation was obtained using Mosaic mutant Analysis with Spatial and Temporal control of Recombination (MASTR).
conclusionsDissociation and reaggregation of fetal kidney cells of different genotypes, with suitable fluorescent markers, provides an efficient way to analyze cell behaviors in chimeric cultures. FGF/Fgfr2 signaling promotes UB cell rearrangements that form the tip domain, similarly to GDNF/Ret signaling.
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