ArticleTransfusion2016
Supernatant protein biomarkers of red blood cell storage hemolysis as determined through an absolute quantification proteomics technology.
Article in Transfusion, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- High-Throughput Metabolomics in Transfusion Medicine.Methods in molecular biology (Clifton, N.J.) · 2026Article
- It's in your blood: The impact of age, sex, genetic factors and exposures on stored red blood cell metabolism.Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis · 2024Review
- Red Blood Cell Omics and Machine Learning in Transfusion Medicine: Singularity Is Near.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2023Review
- Hemorrhagic shock and tissue injury provoke distinct components of trauma-induced coagulopathy in a swine model.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2023Article
- Impact of hemolysis on multi-OMIC pancreatic biomarker discovery to derisk biomarker development in precision medicine studies.Scientific reports · 2022Article
- Deciphering the Relationship Between Free and Vesicular Hemoglobin in Stored Red Blood Cell Units.Frontiers in physiology · 2022Article
- Protect, repair, destroy or sacrifice: a role of oxidative stress biology in inter-donor variability of blood storage?Blood transfusion = Trasfusione del sangue · 2019Review
- A comparison of different methods of red blood cell leukoreduction and additive solutions on the accumulation of neutrophil-priming activity during storage.Transfusion · 2018Article
- Systemic hyperfibrinolysis after trauma: a pilot study of targeted proteomic analysis of superposed mechanisms in patient plasma.The journal of trauma and acute care surgery · 2018Article
- Purinergic control of red blood cell metabolism: novel strategies to improve red cell storage quality.Blood transfusion = Trasfusione del sangue · 2017Review
- Temperature-dependent haemolytic propensity of CPDA-1 stored red blood cells vs whole blood - Red cell fragility as donor signature on blood units.Blood transfusion = Trasfusione del sangue · 2017Article
- Freeze-dried plasma enhances clot formation and inhibits fibrinolysis in the presence of tissue plasminogen activator similar to pooled liquid plasma.Transfusion · 2017Article
- Correlation of pre-operative plasma protein concentrations in cardiac surgery patients with bleeding outcomes using a targeted quantitative proteomics approach.Proteomics. Clinical applications · 2017Observational
- Cumulative erythrocyte damage in blood storage and relevance to massive transfusions: selective insights into serial morphological and biochemical findings.Blood transfusion = Trasfusione del sangue · 2017Review
- Red blood cell storage time and transfusion: current practice, concerns and future perspectives.Blood transfusion = Trasfusione del sangue · 2017Review
- Red blood cells ageing markers: a multi-parametric analysis.Blood transfusion = Trasfusione del sangue · 2017Article
- Duration of red blood cell storage and inflammatory marker generation.Blood transfusion = Trasfusione del sangue · 2017Review
- Omics markers of the red cell storage lesion and metabolic linkage.Blood transfusion = Trasfusione del sangue · 2017Review
- Unraveling the Gordian knot: red blood cell storage lesion and transfusion outcomes.Blood transfusion = Trasfusione del sangue · 2017Review
- Metabolic Linkage and Correlations to Storage Capacity in Erythrocytes from Glucose 6-Phosphate Dehydrogenase-Deficient Donors.Frontiers in medicine · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
backgroundLaboratory technologies have highlighted the progressive accumulation of the so-called "storage lesion," a wide series of alterations to stored red blood cells (RBCs) that may affect the safety and effectiveness of the transfusion therapy. New improvements in the field are awaited to ameliorate this lesion, such as the introduction of washing technologies in the cell processing pipeline. Laboratory studies that have tested such technologies so far rely on observational qualitative or semiquantitative techniques. STUDY DESIGN AND
methodsA state-of-the-art quantitative proteomics approach utilizing quantitative concatamers (QconCAT) was used to simultaneously monitor fluctuations in the abundance of 114 proteins in AS-3 RBC supernatants (n = 5; 11 time points, including before and after leukoreduction, at 3 hours, on Days 1 and 2, and weekly sampling from Day 7 through Day 42).
resultsLeukoreduction-dependent depletion of plasma proteins was observed at the earliest time points. A subset of proteins showed very high linear correlation (r(2) > 0.9) not only with storage time, but also with absolute levels of hemoglobin α1 and β, a proxy for RBC hemolysis and vesiculation. Linear regression was performed to describe the temporal relationship between these proteins. Our findings suggest a role for supernatant glyceraldehyde-3-phosphate dehydrogenase; peroxiredoxin-1, -2, and -6; carbonic anhydrase-1 and -2; selenium binding protein-1; biliverdin reductase; aminolevulinate dehydratase; and catalase as potential biomarkers of RBC quality during storage.
conclusionA targeted proteomics technology revealed novel biomarkers of the RBC storage lesion and promises to become a key analytical readout for the development and testing of alternative cell processing strategies.
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