ArticlePloS one2016
The Phenazine 2-Hydroxy-Phenazine-1-Carboxylic Acid Promotes Extracellular DNA Release and Has Broad Transcriptomic Consequences in Pseudomonas chlororaphis 30-84.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Genetic regulation of biosynthesis and resistance to redox-active phenazines inMicrobiology spectrum · 2026Article
- Unravelling the noncanonical extracellular DNA structures in biofilm and NETosis.Nucleic acids research · 2026Review
- Article
- Review
- New Pseudomonas Bacterial Strains: Biological Activity and Characteristic Properties of Metabolites.Microorganisms · 2023Article
- Bacterial biofilms as an essential component of rhizosphere plant-microbe interactions.Methods in microbiology · 2023Article
- eDNA, Amyloid Fibers and Membrane Vesicles Identified inInternational journal of molecular sciences · 2022Article
- Characterization of the diastaphenazine/izumiphenazine C biosynthetic gene cluster from plant endophyte Streptomyces diastaticus W2.Microbial biotechnology · 2022Article
- Article
- PcsR2 Is a LuxR-Type Regulator That Is Upregulated on Wheat Roots and Is Unique toFrontiers in microbiology · 2020Article
- Phenazine-Producing Rhizobacteria Promote Plant Growth and Reduce Redox and Osmotic Stress in Wheat Seedlings Under Saline Conditions.Frontiers in plant science · 2020Article
- Genetic factors involved in rhizosphere colonization by phytobeneficialComputational and structural biotechnology journal · 2020Review
- Drought-Stress Tolerance in Wheat Seedlings Conferred by Phenazine-Producing Rhizobacteria.Frontiers in microbiology · 2019Article
- Both toxic and beneficial effects of pyocyanin contribute to the lifecycle of Pseudomonas aeruginosa.Molecular microbiology · 2018Article
- Rhizosphere pseudomonads as probiotics improving plant health.Molecular plant pathology · 2018Review
- Effect of Producing Different Phenazines on Bacterial Fitness and Biological Control inThe plant pathology journal · 2018Article
- Lectin-Like Bacteriocins.Frontiers in microbiology · 2018Review
- Article
- Engineering Pseudomonas for phenazine biosynthesis, regulation, and biotechnological applications: a review.World journal of microbiology & biotechnology · 2017Review
- Pseudomonas chlororaphis Produces Two Distinct R-Tailocins That Contribute to Bacterial Competition in Biofilms and on Roots.Applied and environmental microbiology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enhanced production of 2-hydroxy-phenazine-1-carboxylic acid (2-OH-PCA) by the biological control strain Pseudomonas chlororaphis 30-84 derivative 30-84O* was shown previously to promote cell adhesion and alter the three-dimensional structure of surface-attached biofilms compared to the wild type. The current study demonstrates that production of 2-OH-PCA promotes the release of extracellular DNA, which is correlated with the production of structured biofilm matrix. Moreover, the essential role of the extracellular DNA in maintaining the mass and structure of the 30-84 biofilm matrix is demonstrated. To better understand the role of different phenazines in biofilm matrix production and gene expression, transcriptomic analyses were conducted comparing gene expression patterns of populations of wild type, 30-84O* and a derivative of 30-84 producing only PCA (30-84PCA) to a phenazine defective mutant (30-84ZN) when grown in static cultures. RNA-Seq analyses identified a group of 802 genes that were differentially expressed by the phenazine producing derivatives compared to 30-84ZN, including 240 genes shared by the two 2-OH-PCA producing derivatives, the wild type and 30-84O*. A gene cluster encoding a bacteriophage-derived pyocin and its lysis cassette was upregulated in 2-OH-PCA producing derivatives. A holin encoded in this gene cluster was found to contribute to the release of eDNA in 30-84 biofilm matrices, demonstrating that the influence of 2-OH-PCA on eDNA production is due in part to cell autolysis as a result of pyocin production and release. The results expand the current understanding of the functions different phenazines play in the survival of bacteria in biofilm-forming communities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.