ArticleThe Journal of cell biology1989
Biotin-tubulin incorporates into kinetochore fiber microtubules during early but not late anaphase.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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Who cites it
12 citing papers in PubMed, 37 citations in OpenAlex.
- The perpetual movements of anaphase.Cellular and molecular life sciences : CMLS · 2010Review
- Mammalian CLASP1 and CLASP2 cooperate to ensure mitotic fidelity by regulating spindle and kinetochore function.Molecular biology of the cell · 2006Article
- Kinetochores use a novel mechanism for coordinating the dynamics of individual microtubules.Current biology : CB · 2006Article
- Kinetochore fiber maturation in PtK1 cells and its implications for the mechanisms of chromosome congression and anaphase onset.The Journal of cell biology · 1997Article
- Force generation by microtubule assembly/disassembly in mitosis and related movements.Molecular biology of the cell · 1995Review
- Kinetochore microtubule dynamics and the metaphase-anaphase transition.The Journal of cell biology · 1995Article
- Observation and quantification of individual microtubule behavior in vivo: microtubule dynamics are cell-type specific.The Journal of cell biology · 1993Article
- Poleward kinetochore fiber movement occurs during both metaphase and anaphase-A in newt lung cell mitosis.The Journal of cell biology · 1992Article
- Microinjection of biotin-tubulin into anaphase cells induces transient elongation of kinetochore microtubules and reversal of chromosome-to-pole motion.The Journal of cell biology · 1992Article
- Centrophilin: a novel mitotic spindle protein involved in microtubule nucleation.The Journal of cell biology · 1991Article
- Modulation of microtubule stability by kinetochores in vitro.The Journal of cell biology · 1990Article
- Kinetochores are transported poleward along a single astral microtubule during chromosome attachment to the spindle in newt lung cells.The Journal of cell biology · 1990Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The dynamic behavior of kinetochore fiber microtubules has been examined in PtK1 cells during anaphase of mitosis. Cells in anaphase were injected with biotin-tubulin and, at various intervals after injection, fixed for light or electron microscopic immunolocalization of biotin-tubulin-containing microtubules. When cells in early to mid anaphase were injected with biotin-tubulin and fixed 1-2 min later, fluorescence was observed throughout the spindle, including the region of the kinetochore fibers. Electron microscopy of early to mid anaphase cells, after processing with immunogold methods, revealed both labeled and unlabeled microtubules in the kinetochore fibers; some labeled microtubules contacted the kinetochores. When late anaphase cells were injected with biotin-tubulin, and fixed a few minutes later, little fluorescence was observed in the kinetochore fibers. Electron microscopy confirmed that kinetochore fibers in late anaphase cells were refractory to tubulin incorporation. The results of these experiments demonstrate that the kinetochore fiber incorporates new microtubules during early anaphase but that this incorporation ceases in mid to late anaphase. Thus, microtubule turnover within the kinetochore fiber does not abruptly cease at the onset of anaphase and anaphase kinetochore fiber microtubules are more dynamic than previously suspected.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.