ArticleEcology and evolution2015
A simulation study of sample size for DNA barcoding.
Article in Ecology and evolution, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Prospects for Complete DNA Barcode Coverage of the New Zealand Insect Fauna.Journal of the Royal Society of New Zealand · 2026Article
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- Integrative taxonomy suggests hidden diversity within the fish genus Cyttopsis (Zeiformes, Parazenidae).Journal of fish biology · 2025Article
- Genomic exploration of the journey of Plasmodium vivax in Latin America.PLoS pathogens · 2025Article
- Unveiling ophiuroid biodiversity across North Atlantic habitats via an integrative perspective.Scientific reports · 2024Article
- The first DNA barcode library of Chironomidae from the Tibetan Plateau with an evaluation of the status of the public databases.Ecology and evolution · 2023Article
- Collecting wood core samples from Macassar ebony (MethodsX · 2022Article
- Toward a global DNA barcode reference library of the intolerant nonbiting midge genusEcology and evolution · 2021Article
- A Revised Phylogeny of thePlants (Basel, Switzerland) · 2021Article
- Forensic validation of a panel of 12 SNPs for identification of Mongolian wolf and dog.Scientific reports · 2020Article
- HACSim: an R package to estimate intraspecific sample sizes for genetic diversity assessment using haplotype accumulation curves.PeerJ. Computer science · 2020Article
- The impact of genetic diversity on the accuracy of DNA barcoding to identify species: A study on the genusEcology and evolution · 2019Article
- Incomplete estimates of genetic diversity within species: Implications for DNA barcoding.Ecology and evolution · 2019Review
- Comparison of Methods for Molecular Species Delimitation Across a Range of Speciation Scenarios.Systematic biology · 2018Article
- Mitochondrial variation in subpopulations of Anopheles balabacensis Baisas in Sabah, Malaysia (Diptera: Culicidae).PloS one · 2018Article
- Filling reference gaps via assembling DNA barcodes using high-throughput sequencing-moving toward barcoding the world.GigaScience · 2017Article
- Article
- Evaluation of three molecular markers for identification of European primary parasitoids of cereal aphids and their hyperparasitoids.PloS one · 2017Article
- A DNA Barcode Library for North American Pyraustinae (Lepidoptera: Pyraloidea: Crambidae).PloS one · 2016Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For some groups of organisms, DNA barcoding can provide a useful tool in taxonomy, evolutionary biology, and biodiversity assessment. However, the efficacy of DNA barcoding depends on the degree of sampling per species, because a large enough sample size is needed to provide a reliable estimate of genetic polymorphism and for delimiting species. We used a simulation approach to examine the effects of sample size on four estimators of genetic polymorphism related to DNA barcoding: mismatch distribution, nucleotide diversity, the number of haplotypes, and maximum pairwise distance. Our results showed that mismatch distributions derived from subsamples of ≥20 individuals usually bore a close resemblance to that of the full dataset. Estimates of nucleotide diversity from subsamples of ≥20 individuals tended to be bell-shaped around that of the full dataset, whereas estimates from smaller subsamples were not. As expected, greater sampling generally led to an increase in the number of haplotypes. We also found that subsamples of ≥20 individuals allowed a good estimate of the maximum pairwise distance of the full dataset, while smaller ones were associated with a high probability of underestimation. Overall, our study confirms the expectation that larger samples are beneficial for the efficacy of DNA barcoding and suggests that a minimum sample size of 20 individuals is needed in practice for each population.
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