ArticleBiotechnology for biofuels2016
Highly efficient lipid production in the green alga Parachlorella kessleri: draft genome and transcriptome endorsed by whole-cell 3D ultrastructure.
Article in Biotechnology for biofuels, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 91 citations in OpenAlex.
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- Tapping the microalgal potential: genetic precision and stress-induction for enhanced astaxanthin and biofuel production.Biotechnology for biofuels and bioproducts · 2025Review
- Development of a CRISPR/Cas9-mediated gene-editing method to isolate a mutant of the unicellular green alga Parachlorella kessleri strain NIES-2152 with improved lipid productivity.Biotechnology for biofuels and bioproducts · 2024Article
- Coproduction of lipids and carotenoids by the novel green algaBiotechnology reports (Amsterdam, Netherlands) · 2023Article
- Cellular response ofFrontiers in plant science · 2023Article
- Evaluation of the validity of large-scale serial sectioning TEM for three-dimensional reconstruction of rice mesophyll cells and chloroplasts.Protoplasma · 2022Article
- Morphological bases of phytoplankton energy management and physiological responses unveiled by 3D subcellular imaging.Nature communications · 2021Article
- Coproduction of lipids and extracellular polysaccharides from the novel green algaBiotechnology reports (Amsterdam, Netherlands) · 2020Article
- Characterization of a novel herbicide and antibiotic-resistant Chlorella sp. with an extensive extracellular matrix.Photosynthesis research · 2020Article
- Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application.Frontiers in bioengineering and biotechnology · 2020Review
- Molecular profiling of an oleaginous trebouxiophycean algaBiotechnology for biofuels · 2019Article
- Article
- Cas9-guide RNA ribonucleoprotein-induced genome editing in the industrial green algaBiotechnology for biofuels · 2018Article
- Draft genome sequence and detailed characterization of biofuel production by oleaginous microalgaBiotechnology for biofuels · 2018Article
- Three-dimensional intracellular structure of a whole rice mesophyll cell observed with FIB-SEM.Annals of botany · 2017Article
- Imaging the accumulated intracellular microalgal lipids as a response to temperature stress.3 Biotech · 2017Article
- Genome and transcriptome analysis of surfactin biosynthesis in Bacillus amyloliquefaciens MT45.Scientific reports · 2017Article
- Reactive Oxygen Species-Mediated Cellular Stress Response and Lipid Accumulation in Oleaginous Microorganisms: The State of the Art and Future Perspectives.Frontiers in microbiology · 2017Review
- Deciphering the relationship among phosphate dynamics, electron-dense body and lipid accumulation in the green alga Parachlorella kessleri.Scientific reports · 2016Article
- Strategies for Lipid Production Improvement in Microalgae as a Biodiesel Feedstock.BioMed research international · 2016Review
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlgae have attracted attention as sustainable producers of lipid-containing biomass for food, animal feed, and for biofuels. Parachlorella kessleri, a unicellular green alga belonging to the class Trebouxiophyceae, achieves very high biomass, lipid, and starch productivity levels. However, further biotechnological exploitation has been hampered by a lack of genomic information.
resultsHere, we sequenced the whole genome and transcriptome, and analyzed the behavior of P. kessleri NIES-2152 under lipid production-inducing conditions. The assembly includes 13,057 protein-coding genes in a 62.5-Mbp nuclear genome. Under conditions of sulfur deprivation, lipid accumulation was correlated with the transcriptomic induction of enzymes involved in sulfur metabolism, triacylglycerol (TAG) synthesis, autophagy, and remodeling of light-harvesting complexes.
conclusionsThree-dimensional transmission electron microscopy (3D-TEM) revealed extensive alterations in cellular anatomy accompanying lipid hyperaccumulation. The present 3D-TEM results, together with transcriptomic data support the finding that upregulation of TAG synthesis and autophagy are potential key mediators of the hyperaccumulation of lipids under conditions of nutrient stress.
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