Evidence map›Paper›PMID 26810244›Full record

ReviewDifferentiation; research in biological diversity

Diethylstilbestrol-induced mouse hypospadias: "window of susceptibility".

Adriane Watkins Sinclair, Mei Cao, Laurence Baskin, Gerald R Cunha

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Differentiation; research in biological diversity. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Article
  6. Atrazine induces penis abnormalities including hypospadias in mice.Journal of developmental origins of health and disease · 2020
    Article
  7. Discrete Hedgehog Factor Expression and Action in the Developing Phallus.International journal of molecular sciences · 2020
    Article
  8. Comparative Morphology of the Penis and Clitoris in Four Species of Moles (Talpidae).Journal of experimental zoology. Part B, Molecular and developmental evolution · 2017
    Article
  9. Mouse hypospadias: A critical examination and definition.Differentiation; research in biological diversity · 2016
    Article
  10. Review
  11. Anatomy of the mouse penis and internal prepuce.Differentiation; research in biological diversity
    Review
  12. Estrogens and development of the mouse and human external genitalia.Differentiation; research in biological diversity
    Review
  13. Development of the human prepuce and its innervation.Differentiation; research in biological diversity
    Article
  14. Reproductive tract biology: Of mice and men.Differentiation; research in biological diversity
    Review
  15. Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Adriane Watkins SinclairDepartment of Urology, University of California San Francisco, 400 Parnassus Avenue, Box A610, San Francisco, CA 94143, United States.
Mei CaoDepartment of Urology, University of California San Francisco, 400 Parnassus Avenue, Box A610, San Francisco, CA 94143, United States.
Laurence BaskinDepartment of Urology, University of California San Francisco, 400 Parnassus Avenue, Box A610, San Francisco, CA 94143, United States.
Gerald R CunhaDepartment of Urology, University of California San Francisco, 400 Parnassus Avenue, Box A610, San Francisco, CA 94143, United States. Electronic address: gerald.cunha@ucsf.edu.

Funding

UCSF KURe Career Development ProgramK12DK083021 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI BASKIN, LAURENCE, CARROLL, PETER R · 2008 to 2017
$5.9M
Hypospadias Differentiation and Endocrine DisruptorsR01DK058105 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI BASKIN, LAURENCE · 2003 to 2015
$2.7M
NIDDK NIH HHS K12 DK083021NIDDK NIH HHS R01 DK058105NIDDK NIH HHS R01DK0581050
6 · The paper itself

Abstract

This review presents published and novel results that define the programming window for diethylstilbestrol (DES)-induced abnormal development of the mouse penis. These data indicate that DES has its greatest effect during the period of most intense penile morphogenesis, namely postnatal days 0-15 (P0-P15). Pregnant mice and their neonatal pups were injected subcutaneously with 200 ng/gbw DES every other day from embryonic day 12-18 (DES E12-E18), postnatal day 0-10 (DES P0-P10), embryonic day 12 to postnatal day 10 (DES E12-P10), postnatal day 5-15 (DES P5-P15), and postnatal day 10-20 (DES P10-P20). Aged-matched controls received sesame oil vehicle. After euthanasia at 10, 15, 20 and 60 days, penises were analyzed by gross morphology, histology and morphometry. Penises of all 5 groups of DES-treated mice were reduced in size, which was confirmed by morphometric analysis of internal penile structures. The most profound effects were seen in the DES E12-P10, DES P0-P10, and DES P5-P15 groups, thus defining a DES "programming window". For all parameters, DES treatment from P10 to P20 showed the most mild of effects. Adverse effects of DES on the MUMP cartilage and erectile bodies observed shortly after the last DES injection reverted to normality in the DES P5-P15, but not in the E12-P10 and P0-P10 groups, in which MUMP cartilage and erectile body malformations persisted into adulthood, again emphasizing a "window of susceptibility" in the early neonatal period.

Indexed as

AnimalsAnimals, NewbornDiethylstilbestrolDisease SusceptibilityEstrogens, Non-SteroidalFemaleHumansHypospadiasMaleMiceMorphogenesisPenisPregnancyDiethylstilbestrolEstrogens, Non-SteroidalDiethylstilbestrolEstrogenMouse penile developmentUrethra

Identifiers

PMID26810244
PMCPMC4803596

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.