ArticleMicrobial cell factories2016
Reorganization of a synthetic microbial consortium for one-step vitamin C fermentation.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Article
- Efficient Production of 2-Keto-l-Gulonic Acid via One-Step Fermentation UsingMicroorganisms · 2026Article
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- A dual light-controlled co-culture system enables the regulation of population composition.Synthetic and systems biotechnology · 2025Article
- Strategies and tools to construct stable and efficient artificial coculture systems as biosynthetic platforms for biomass conversion.Biotechnology for biofuels and bioproducts · 2024Review
- A systematic discussion and comparison of the construction methods of synthetic microbial community.Synthetic and systems biotechnology · 2024Review
- Untargeted urine metabolomics suggests that ascorbic acid may serve as a promising biomarker for reduced feed intake in rabbits.Scientific reports · 2024Article
- L-gulono-γ-lactone Oxidase, the Key Enzyme for L-Ascorbic Acid Biosynthesis.Current issues in molecular biology · 2024Review
- Draft genome sequences of twoMicrobiology resource announcements · 2024Article
- A structured evaluation of genome-scale constraint-based modeling tools for microbial consortia.PLoS computational biology · 2023Article
- Design and construction of microbial cell factories based on systems biology.Synthetic and systems biotechnology · 2023Review
- iTRAQ-based proteomics analysis ofFrontiers in microbiology · 2023Article
- Genetic and biochemical strategies for regulation of L-ascorbic acid biosynthesis in plants through the L-galactose pathway.Frontiers in plant science · 2023Review
- Artificial microbial consortia for bioproduction processes.Engineering in life sciences · 2023Review
- Microbial Interactions in a Vitamin C Industrial Fermentation System: Novel Insights and Perspectives.Applied and environmental microbiology · 2022Review
- The industrial versatility of Gluconobacter oxydans: current applications and future perspectives.World journal of microbiology & biotechnology · 2022Review
- Multiscale engineering of microbial cell factories: A step forward towards sustainable natural products industry.Synthetic and systems biotechnology · 2022Review
- Construction of Environmental Synthetic Microbial Consortia: Based on Engineering and Ecological Principles.Frontiers in microbiology · 2022Review
- The l-rhamnose-dependent regulator RhaS and its target promoters fromFrontiers in microbiology · 2022Article
- Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundIn the industry, the conventional two-step fermentation method was used to produce 2-keto-L-gulonic acid (2-KGA), the precursor of vitamin C, by three strains, namely, Gluconobacter oxydans, Bacillus spp. and Ketogulonicigenium vulgare. Despite its high production efficiency, the long incubation period and an additional second sterilization process inhibit the further development. Therefore, we aimed to reorganize a synthetic consortium of G. oxydans and K. vulgare for one-step fermentation of 2-KGA and enhance the symbiotic interaction between microorganisms to perform better.
resultsDuring the fermentation, competition for sorbose of G. oxydans arose when co-cultured with K. vulgare. In this study, the competition between the two microbes was alleviated and their mutualism was enhanced by deleting genes involved in sorbose metabolism of G. oxydans. In the engineered synthetic consortium (H6 + Kv), the yield of 2-KGA (mol/mol) against D-sorbitol reached 89.7 % within 36 h, increased by 29.6 %. Furthermore, metabolomic analysis was used to verify the enhancement of the symbiotic relationship and to provide us potential strategies for improving the synthetic consortium. Additionally, a significant redistribution of metabolism occurred by co-culturing the K. vulgare with the engineered G. oxydans, mainly reflected in the increased TCA cycle, purine, and fatty acid metabolism.
conclusionsWe reorganized and optimized a synthetic consortium of G. oxydans and K. vulgare to produce 2-KGA directly from D-sorbitol. The yield of 2-KGA was comparable to that of the conventional two-step fermentation. The metabolic interaction between the strains was further investigated by metabolomics, which verified the enhancement of the mutualism between the microbes and gave us a better understanding of the synthetic consortium.
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