ArticlePloS one2016
Accurate Identification of Common Pathogenic Nocardia Species: Evaluation of a Multilocus Sequence Analysis Platform and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The species distribution and antimicrobial resistance profiles of Nocardia species in China: A systematic review and meta-analysis.PLoS neglected tropical diseases · 2023Pooled it
- Genetic Diversity ofAnimals : an open access journal from MDPI · 2025Article
- Application of MALDI-TOF mass spectrometry for identification of Nocardia species.BMC microbiology · 2024Article
- PersistentInfectious medicine · 2023Article
- Clinical Characteristics of Nocardiosis: a Multicenter Retrospective Study in Korea.Infection & chemotherapy · 2023Article
- Molecular identification and antimicrobial resistance pattern of Nocardia isolated from 14 diseased dogs and cats.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Updated Review onClinical microbiology reviews · 2022Review
- Article
- Microbial risk assessment ofComputational and structural biotechnology journal · 2021Article
- Clinical Nocardia species: Identification, clinical characteristics, and antimicrobial susceptibility in Shandong, China.Bosnian journal of basic medical sciences · 2020Article
- Assessment of VITEK® MS IVD database V3.0 for identification of Nocardia spp. using two culture media and comparing direct smear and protein extraction procedures.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
- Observational
- Genome Sequences of Three Nocardia cyriacigeorgica Strains and One Nocardia asteroides Strain.Microbiology resource announcements · 2019Article
- Isolation and characterization of bacteriophage NTR1 infectious for Nocardia transvalensis and other Nocardia species.Virus genes · 2019Article
- Evaluation of the Vitek MS v3.0 Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry System for Identification of Mycobacterium and Nocardia Species.Journal of clinical microbiology · 2018Article
- The Complexities of Nocardia Taxonomy and Identification.Journal of clinical microbiology · 2018Review
- Shortcomings of the Commercial MALDI-TOF MS Database and Use of MLSA as an Arbiter in the Identification of Nocardia Species.Frontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Species identification of Nocardia is not straightforward due to rapidly evolving taxonomy, insufficient discriminatory power of conventional phenotypic methods and also of single gene locus analysis including 16S rRNA gene sequencing. Here we evaluated the ability of a 5-locus (16S rRNA, gyrB, secA1, hsp65 and rpoB) multilocus sequence analysis (MLSA) approach as well as that of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) in comparison with sequencing of the 5'-end 606 bp partial 16S rRNA gene to provide identification of 25 clinical isolates of Nocardia. The 5'-end 606 bp 16S rRNA gene sequencing successfully assigned 24 of 25 (96%) clinical isolates to species level, namely Nocardia cyriacigeorgica (n = 12, 48%), N. farcinica (n = 9, 36%), N. abscessus (n = 2, 8%) and N. otitidiscaviarum (n = 1, 4%). MLSA showed concordance with 16S rRNA gene sequencing results for the same 24 isolates. However, MLSA was able to identify the remaining isolate as N. wallacei, and clustered N. cyriacigeorgica into three subgroups. None of the clinical isolates were correctly identified to the species level by MALDI-TOF MS analysis using the manufacturer-provided database. A small "in-house" spectral database was established incorporating spectra of five clinical isolates representing the five species identified in this study. After complementation with the "in-house" database, of the remaining 20 isolates, 19 (95%) were correctly identified to species level (score ≥ 2.00) and one (an N. abscessus strain) to genus level (score ≥ 1.70 and < 2.00). In summary, MLSA showed superior discriminatory power compared with the 5'-end 606 bp partial 16S rRNA gene sequencing for species identification of Nocardia. MALDI-TOF MS can provide rapid and accurate identification but is reliant on a robust mass spectra database.
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