ArticlePloS one2016
N-Acetyl Cysteine Depletes Reactive Oxygen Species and Prevents Dental Monomer-Induced Intrinsic Mitochondrial Apoptosis In Vitro in Human Dental Pulp Cells.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 52 citations in OpenAlex.
- Dentin tubule penetration of a calcium silicate-based sealer after removal of calcium hydroxide or N-acetylcysteine medicaments: an in vitro study.BMC oral health · 2026Article
- Assessment of physicochemical properties and biological responses of chemically surface-modified microcapsules for self-healing dental restorative materials.Materials & design · 2025Article
- Impact of Thermo-Responsive N-Acetylcysteine Hydrogel on Dermal Wound Healing and Oral Ulcer Regeneration.International journal of molecular sciences · 2024Article
- Mitochondrial membrane potential acts as a retrograde signal to regulate cell cycle progression.Life science alliance · 2023Article
- Evaluation of oxidative stress cycle in healthy and inflamed dental pulp tissue: a laboratory investigation.Clinical oral investigations · 2023Article
- In vitro cytotoxicity of resin cement and its influence on the expression of antioxidant genes.Acta odontologica latinoamericana : AOL · 2023Article
- Comparison of Cytotoxicity and Genotoxicity in Three Types of Indirect Restorative Materials on Human Periodontal Stem Cells.Oral health & preventive dentistry · 2023Article
- Effect of N-Acetylcysteine on Sleep: Impacts of Sex and Time of Day.Antioxidants (Basel, Switzerland) · 2023Article
- Article
- Akt-GSK3β-mPTP pathway regulates the mitochondrial dysfunction contributing to odontoblasts apoptosis induced by glucose oxidative stress.Cell death discovery · 2022Article
- Comparative analysis of leaching residual monomer and biological effects of four types of conventional and CAD/CAM dental polymers: an in vitro study.Clinical oral investigations · 2022Article
- Prosthetic Materials Used for Implant-Supported Restorations and Their Biochemical Oral Interactions: A Narrative Review.Materials (Basel, Switzerland) · 2022Review
- Article
- The role of reactive oxygen species in the immunity induced by nano-pulse stimulation.Scientific reports · 2021Article
- Effects of N-acetyl cysteine on mitochondrial ROS, mitochondrial dynamics, and inflammation on lipopolysaccharide-treated human apical papilla cells.Clinical oral investigations · 2021Article
- Upregulation of V-ATPase by STAT3 Activation Promotes Anoikis Resistance and Tumor Metastasis.Journal of Cancer · 2021Article
- N-Acetylcysteine: A Review of Clinical Usefulness (an Old Drug with New Tricks).Journal of nutrition and metabolism · 2021Review
- The Effect of Selected Dental Materials Used in Conservative Dentistry, Endodontics, Surgery, and Orthodontics as Well as during the Periodontal Treatment on the Redox Balance in the Oral Cavity.International journal of molecular sciences · 2020Review
- N-Acetyl Cysteine Modulates the Inflammatory and Oxidative Stress Responses of Rescued Growth-Arrested Dental Pulp Microtissues Exposed to TEGDMA in ECM.International journal of molecular sciences · 2020Article
- Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo investigate the involvement of intrinsic mitochondrial apoptosis in dental monomer-induced cytotoxicity and the influences of N-acetyl cysteine (NAC) on this process.
methodsHuman dental pulp cells (hDPCs) were exposed to several dental monomers in the absence or presence of NAC, and cell viability, intracellular redox balance, morphology and function of mitochondria and key indicators of intrinsic mitochondrial apoptosis were evaluated using various commercial kits.
resultsDental monomers exerted dose-dependent cytotoxic effects on hDPCs. Concomitant to the over-production of reactive oxygen species (ROS) and depletion of glutathione (GSH), differential changes in activities of superoxide dismutase, glutathione peroxidase, and catalase were detected. Apoptosis, as indicated by positive Annexin V/propidium iodide (PI) staining and activation of caspase-3, was observed after dental monomer treatment. Dental monomers impaired the morphology and function of mitochondria, and induced intrinsic mitochondrial apoptosis in hDPCs via up-regulation of p53, Bax and cleaved caspase-3, and down-regulation of Bcl-2. NAC restored cell viability, relieved oxidative stress and blocked the apoptotic effects of dental monomers.
conclusionsDental monomers induced oxidative stress and mitochondrial intrinsic apoptosis in hDPCs. NAC could reduce the oxidative stress and thus protect hDPCs against dental monomer-induced apoptosis.
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Registered trials
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