ArticleMolecular and cellular endocrinology2016
HNF1 regulates critical processes in the human epididymis epithelium.
Article in Molecular and cellular endocrinology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Hepatocyte nuclear factors dynamically regulate triglyceride metabolic reprogramming in metabolic dysfunction-associated steatotic liver disease: Mechanisms and implications.World journal of hepatology · 2025Review
- CFTR Gene Regulation in Human Pancreatic Duct, Bile Duct and Sweat Gland Epithelial Cells.Journal of cellular and molecular medicine · 2025Article
- Tissue-Specific Regulation ofInternational journal of molecular sciences · 2023Review
- BACH1, the master regulator of oxidative stress, has a dual effect on CFTR expression.The Biochemical journal · 2021Article
- Transcriptome analysis of turkey (Meleagris gallopavo) reproductive tract revealed key pathways regulating spermatogenesis and post-testicular sperm maturation.Poultry science · 2020Article
- An atlas of human proximal epididymis reveals cell-specific functions and distinct roles for CFTR.Life science alliance · 2020Article
- A functional genomics approach to investigate the differentiation of iPSCs into lung epithelium at air-liquid interface.Journal of cellular and molecular medicine · 2020Article
- The FOXA1 transcriptional network coordinates key functions of primary human airway epithelial cells.American journal of physiology. Lung cellular and molecular physiology · 2020Article
- Transcriptional networks in the human epididymis.Andrology · 2019Review
- Cell-Selective Regulation of CFTR Gene Expression: Relevance to Gene Editing Therapeutics.Genes · 2019Review
- A novel transcriptional network for the androgen receptor in human epididymis epithelial cells.Molecular human reproduction · 2018Article
- Cell function and identity revealed by comparative scRNA-seq analysis in human nasal, bronchial and epididymis epithelia.European journal of cell biologyArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The luminal environment of the epididymis participates in sperm maturation and impacts male fertility. It is dependent on the coordinated expression of many genes encoding proteins with a role in epithelial transport. We identified cis-regulatory elements for critical genes in epididymis function, by mapping open chromatin genome-wide in human epididymis epithelial (HEE) cells. Bioinformatic predictions of transcription factors binding to the regulatory elements suggested an important role for hepatocyte nuclear factor 1 (HNF1) in the transcriptional program of these cells. Chromatin immunoprecipitation and deep sequencing (ChIP-seq) revealed HNF1 target genes in HEE cells. In parallel, the contribution of HNF1 to the transcriptome of HEE cells was determined by RNA-seq, following siRNA-mediated depletion of both HNF1α and HNF1β transcription factors. Repression of these factors caused differential expression of 1892 transcripts (902 were downregulated and 990 upregulated) in comparison to non-targeting siRNAs. Differentially expressed genes with HNF1 ChIP-seq peaks within 20 kb were subject to gene ontology process enrichment analysis. Among the most significant processes associated with down-regulated genes were epithelial transport of water, phosphate and bicarbonate, all critical processes in epididymis epithelial function. Measurements of intracellular pH (pHi) confirmed a role for HNF1 in regulating the epididymis luminal environment.
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Registered trials
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