Evidence map›Paper›PMID 26808359›Full record

ArticleJournal of virological methods2016

Standardization of a cytometric p24-capture bead-assay for the detection of main HIV-1 subtypes.

Mélanie Merbah, Sayali Onkar, Jean-Charles Grivel, Christophe Vanpouille, Angélique Biancotto, Lydia Bonar, Eric Sanders-Buell, Gustavo Kijak, Nelson Michael, Merlin Robb and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virological methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Diagnostic applications of microsphere-based flow cytometry: A review.Experimental biology and medicine (Maywood, N.J.) · 2022
    Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Mélanie MerbahU.S. Military HIV Research Program, Walter Reed Army Institute of Research, MD, USA; Henry M. Jackson Foundation for the Advancement of Military Medicine, MD, USA. Electronic address: mmerbah@hivresearch.org.
Sayali OnkarU.S. Military HIV Research Program, Walter Reed Army Institute of Research, MD, USA; Henry M. Jackson Foundation for the Advancement of Military Medicine, MD, USA. Electronic address: sonkar@hivresearch.org.
Jean-Charles GrivelEunice Kennedy Shriver National Institute of Child Health and Human Development, Program of Physical Biology, National Institutes of Health, MD, USA. Electronic address: jgrivel@sidra.org.
Christophe VanpouilleEunice Kennedy Shriver National Institute of Child Health and Human Development, Program of Physical Biology, National Institutes of Health, MD, USA. Electronic address: vanpouic@mail.nih.gov.
Angélique BiancottoCenter for Human Immunology, Autoimmunity, and Inflammation, National Institutes of Health, Bethesda, USA. Electronic address: biancoa@nhlbi.nih.gov.
Lydia BonarU.S. Military HIV Research Program, Walter Reed Army Institute of Research, MD, USA; Henry M. Jackson Foundation for the Advancement of Military Medicine, MD, USA. Electronic address: lbonar@hivresearch.org.
Eric Sanders-BuellU.S. Military HIV Research Program, Walter Reed Army Institute of Research, MD, USA; Henry M. Jackson Foundation for the Advancement of Military Medicine, MD, USA. Electronic address: esanders-buell@hivresearch.org.
Gustavo KijakU.S. Military HIV Research Program, Walter Reed Army Institute of Research, MD, USA; Henry M. Jackson Foundation for the Advancement of Military Medicine, MD, USA. Electronic address: gkijak@hivresearch.org.
Nelson MichaelU.S. Military HIV Research Program, Walter Reed Army Institute of Research, MD, USA. Electronic address: nmichael@hivresearch.org.
Merlin RobbU.S. Military HIV Research Program, Walter Reed Army Institute of Research, MD, USA; Henry M. Jackson Foundation for the Advancement of Military Medicine, MD, USA. Electronic address: mrobb@hivresearch.org.
Jerome H KimU.S. Military HIV Research Program, Walter Reed Army Institute of Research, MD, USA. Electronic address: jerome.kim@ivi.int.
Sodsai TovanabutraU.S. Military HIV Research Program, Walter Reed Army Institute of Research, MD, USA; Henry M. Jackson Foundation for the Advancement of Military Medicine, MD, USA. Electronic address: stovanabutra@hivresearch.org.
Agnès-Laurence ChenineU.S. Military HIV Research Program, Walter Reed Army Institute of Research, MD, USA; Henry M. Jackson Foundation for the Advancement of Military Medicine, MD, USA. Electronic address: achenine@hivresearch.org.
Henry M. Jackson Foundation · USNational Institutes of Health · USWalter Reed Army Institute of Research · US

Funding

Intramural NIH HHS Z99 HD999999
6 · The paper itself

Abstract

The prevailing method to assess HIV-1 replication and infectivity is to measure the production of p24 Gag protein by enzyme-linked immunosorbent assay (ELISA). Since fluorescent bead-based technologies offer a broader dynamic range and higher sensitivity, this study describes a p24 capture Luminex assay capable of detecting HIV-1 subtypes A-D, circulating recombinant forms (CRF) CRF01_AE and CRF02_AG, which together are responsible for over 90% of HIV-1 infections worldwide. The success of the assay lies in the identification and selection of a cross-reactive capture antibody (clone 183-H12-5C). Fifty-six isolates that belonged to six HIV-1 subtypes and CRFs were successfully detected with p-values below 0.021; limits of detection ranging from 3.7 to 3 × 104 pg/ml. The intra- and inter-assay variation gave coefficient of variations below 6 and 14%, respectively. The 183-bead Luminex assay also displayed higher sensitivity of 91% and 98% compared to commercial p24 ELISA and a previously described Luminex assay. The p24 concentrations measured by the 183-bead Luminex assay showed a significant correlation (R=0.92, p<0.0001) with the data obtained from quantitative real time PCR. This newly developed p24 assay leverages the advantages of the Luminex platform, which include smaller sample volume and simultaneous detection of up to 500 analytes in a single sample, and delivers a valuable tool for the field.

Indexed as

Enzyme-Linked Immunosorbent AssayFluorescent Antibody Technique, IndirectHIV-1Biological AssayHIV Core Protein p24HIV InfectionsMultiplex Polymerase Chain ReactionReal-Time Polymerase Chain ReactionSensitivity and SpecificityHIV Core Protein p24DetectionHIV-1Multiplex assayMulti-subtypep24

Identifiers

PMID26808359
PMCPMC5812480
OpenAlexW2257178198

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.