ArticlePloS one2016
Induction of Viable but Nonculturable State in Rhodococcus and Transcriptome Analysis Using RNA-seq.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Cell Disruption and RNA Extraction fromInternational journal of molecular sciences · 2026Article
- FeBiofilm · 2026Review
- Phenotypic and transcriptional characterization ofFrontiers in microbiology · 2024Article
- Mechanistic insights into sulfur source-driven physiological responses and metabolic reorganization in the fuel-biodesulfurizingApplied and environmental microbiology · 2023Article
- Insights into the biodegradation of polycaprolactone through genomic analysis of two plastic-degradingFrontiers in microbiology · 2023Article
- Transcriptomic analysis ofFrontiers in microbiology · 2023Article
- Wake Up! Resuscitation of Viable but Nonculturable Bacteria: Mechanism and Potential Application.Foods (Basel, Switzerland) · 2022Review
- Overview of VBNC, a survival strategy for microorganisms.3 Biotech · 2022Review
- Enzymatic properties and biological activity of resuscitation-promoting factor B ofFrontiers in microbiology · 2022Article
- VBNC, previously unrecognized in the life cycle ofJournal of oral microbiology · 2022Review
- Transcriptomic analysis of Rhodococcus opacus R7 grown on polyethylene by RNA-seq.Scientific reports · 2021Article
- Responses to Ecopollutants and Pathogenization Risks of SaprotrophicPathogens (Basel, Switzerland) · 2021Review
- Transcriptome-Guided Insights Into Plastic Degradation by the Marine Bacterium.Frontiers in microbiology · 2021Article
- Transcriptomic Analysis ofFrontiers in microbiology · 2020Article
- Metatranscriptomic and comparative genomic insights into resuscitation mechanisms during enrichment culturing.Microbiome · 2018Article
- Survival strategies of Escherichia coli and Vibrio spp.: contribution of the viable but nonculturable phenotype to their stress-resistance and persistence in adverse environments.World journal of microbiology & biotechnology · 2017Review
- Low temperature-induced viable but not culturable state of Ralstonia eutropha and its relationship to accumulated polyhydroxybutyrate.FEMS microbiology letters · 2016Article
- Transcriptomic analysis on the formation of the viable putative non-culturable state of beer-spoilage Lactobacillus acetotolerans.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viable but nonculturable (VBNC) bacteria, which maintain the viability with loss of culturability, universally exist in contaminated and non-contaminated environments. In this study, two strains, Rhodococcus sp. TG13 and TN3, which were isolated from PCB-contaminated sediment and non-contaminated sediment respectively, were investigated under low temperature and oligotrophic conditions. The results indicated that the two strains TG13 and TN3 could enter into the VBNC state with different incubation times, and could recover culturability by reversal of unfavourable factors and addition of resuscitation-promoting factor (Rpf), respectively. Furthermore, the gene expression variations in the VBNC response were clarified by Illumina high throughput RNA-sequencing. Genome-wide transcriptional analysis demonstrated that up-regulated genes in the VBNC cells of the strain TG13 related to protein modification, ATP accumulation and RNA polymerase, while all differentially expressed genes (DEGs) in the VBNC cells of the strain TN3 were down-regulated. However, the down-regulated genes in both the two strains mainly encoded NADH dehydrogenase subunit, catalase, oxidoreductase, which further verified that cold-induced loss of ability to defend oxidative stress may play an important role in induction of the VBNC state. This study further verified that the molecular mechanisms underlying the VBNC state varied with various bacterial species. Study on the VBNC state of non-pathogenic bacteria will provide new insights into the limitation of environmental micro-bioremediation and the cultivation of unculturable species.
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