ArticleJournal of molecular and cellular cardiology2016
Increased fibroblast chymase production mediates procollagen autophagic digestion in volume overload.
Article in Journal of molecular and cellular cardiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- Characterizing SV40-hTERT Immortalized Human Lung Microvascular Endothelial Cells as Model System for Mechanical Stretch-Induced Lung Injury.International journal of molecular sciences · 2025Article
- Heart Failure in Menopause: Treatment and New Approaches.International journal of molecular sciences · 2022Review
- The effects of cardiac stretch on atrial fibroblasts: analysis of the evidence and potential role in atrial fibrillation.Cardiovascular research · 2022Review
- Flagellin Alleviates Airway Allergic Response by Stabilizing Eosinophils through Modulating Oxidative Stress.Journal of innate immunity · 2021Article
- Reversal of angiotensin-(1-12)-caused positive modulation on left ventricular contractile performance in heart failure: Assessment by pressure-volume analysis.International journal of cardiology · 2020Article
- Estrogen modulates the differential expression of cardiac myocyte chymase isoforms and diastolic function.Molecular and cellular biochemistry · 2019Article
- Chymase uptake by cardiomyocytes results in myosin degradation in cardiac volume overload.Heliyon · 2019Article
- Activation of the Human Angiotensin-(1-12)-Chymase Pathway in Rats With Human Angiotensinogen Gene Transcripts.Frontiers in cardiovascular medicine · 2019Article
- Chymase inhibitors for the treatment of cardiac diseases: a patent review (2010-2018).Expert opinion on therapeutic patents · 2018Review
- Critical role of the chymase/angiotensin-(1-12) axis in modulating cardiomyocyte contractility.International journal of cardiology · 2018Article
- Blunting of estrogen modulation of cardiac cellular chymase/RAS activity and function in SHR.Journal of cellular physiology · 2018Article
- miR199a-5p inhibits hepatic insulin sensitivity via suppression of ATG14-mediated autophagy.Cell death & disease · 2018Article
- Multifunctional Role of Chymase in Acute and Chronic Tissue Injury and Remodeling.Circulation research · 2018Review
- Mouse Mast Cell Protease 4 Deletion Protects Heart Function and Survival After Permanent Myocardial Infarction.Frontiers in pharmacology · 2018Article
- Dual inhibition of cathepsin G and chymase reduces myocyte death and improves cardiac remodeling after myocardial ischemia reperfusion injury.Basic research in cardiology · 2017Article
- Chymase-dependent production of angiotensin II: an old enzyme in old hearts.American journal of physiology. Heart and circulatory physiology · 2017Article
- Novel Cardiac Intracrine Mechanisms Based on Ang-(1-12)/Chymase Axis Require a Revision of Therapeutic Approaches in Human Heart Disease.Current hypertension reports · 2017Review
- Intracrine angiotensin II functions originate from noncanonical pathways in the human heart.American journal of physiology. Heart and circulatory physiology · 2016Review
- Blunting of cardioprotective actions of estrogen in female rodent heart linked to altered expression of cardiac tissue chymase and ACE2.Journal of the renin-angiotensin-aldosterone system : JRAASArticle
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8 authors at 3 institutions in 1 country.
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Abstract
backgroundPrevious work has identified mast cells as the major source of chymase largely associated with a profibrotic phenotype. We recently reported increased fibroblast autophagic procollagen degradation in a rat model of pure volume overload (VO). Here we demonstrate a connection between increased fibroblast chymase production and autophagic digestion of procollagen in the pure VO of aortocaval fistula (ACF) in the rat. METHODS AND
resultsIsolated LV fibroblasts taken from 4 and 12week ACF Sprague-Dawley rats have significant increases in chymase mRNA and chymase activity. Increased intracellular chymase protein is documented by immunocytochemistry in the ACF fibroblasts compared to cells obtained from age-matched sham rats. To implicate VO as a stimulus for chymase production, we show that isolated adult rat LV fibroblasts subjected to 24h of 20% cyclical stretch induces chymase mRNA and protein production. Exogenous chymase treatment of control isolated adult cardiac fibroblasts demonstrates that chymase is internalized through a dynamin-dependent mechanism. Chymase treatment leads to an increased formation of autophagic vacuoles, LC3-II production, autophagic flux, resulting in increased procollagen degradation. Chymase inhibitor treatment reduces cyclical stretch-induced autophagy in isolated cardiac fibroblasts, demonstrating chymase's role in autophagy induction.
conclusionIn a pure VO model, chymase produced in adult cardiac fibroblasts leads to autophagic degradation of newly synthesized intracellular procollagen I, suggesting a new role of chymase in extracellular matrix degradation.
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