Evidence map›Paper›PMID 26806181›Full record

ArticleNature communications2016

A novel mechanism for the biogenesis of outer membrane vesicles in Gram-negative bacteria.

Sandro Roier, Franz G Zingl, Fatih Cakar, Sanel Durakovic, Paul Kohl, Thomas O Eichmann, Lisa Klug, Bernhard Gadermaier, Katharina Weinzerl, Ruth Prassl and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 312 papers.

0numbers the graph read from it
0cells of the map it votes in
312citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

312 citing papers in PubMed.

  1. Review
  2. Virulence · 2026
    Review
  3. Review
  4. Article
  5. Review
  6. Article
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  15. Article
  16. Review
  17. Review
  18. Review
  19. Article
  20. Article

252 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors.

Sandro RoierInstitute of Molecular Biosciences, University of Graz, NAWI Graz, BioTechMed-Graz, Humboldtstraße 50, A-8010 Graz, Austria.
Franz G ZinglInstitute of Molecular Biosciences, University of Graz, NAWI Graz, BioTechMed-Graz, Humboldtstraße 50, A-8010 Graz, Austria.
Fatih CakarInstitute of Molecular Biosciences, University of Graz, NAWI Graz, BioTechMed-Graz, Humboldtstraße 50, A-8010 Graz, Austria.
Sanel DurakovicInstitute of Molecular Biosciences, University of Graz, NAWI Graz, BioTechMed-Graz, Humboldtstraße 50, A-8010 Graz, Austria.
Paul KohlInstitute of Molecular Biosciences, University of Graz, NAWI Graz, BioTechMed-Graz, Humboldtstraße 50, A-8010 Graz, Austria.
Thomas O EichmannInstitute of Molecular Biosciences, University of Graz, NAWI Graz, BioTechMed-Graz, Humboldtstraße 50, A-8010 Graz, Austria.
Lisa KlugInstitute of Biochemistry, Graz University of Technology, NAWI Graz, BioTechMed-Graz, Petersgasse 12/2, A-8010 Graz, Austria.
Bernhard GadermaierInstitute of Molecular Biosciences, University of Graz, NAWI Graz, BioTechMed-Graz, Humboldtstraße 50, A-8010 Graz, Austria.
Katharina WeinzerlInstitute of Molecular Biosciences, University of Graz, NAWI Graz, BioTechMed-Graz, Humboldtstraße 50, A-8010 Graz, Austria.
Ruth PrasslInstitute of Biophysics, Medical University of Graz, BioTechMed-Graz, Harrachgasse 21, A-8010 Graz, Austria.
Achim LassInstitute of Molecular Biosciences, University of Graz, NAWI Graz, BioTechMed-Graz, Humboldtstraße 50, A-8010 Graz, Austria.
Günther DaumInstitute of Biochemistry, Graz University of Technology, NAWI Graz, BioTechMed-Graz, Petersgasse 12/2, A-8010 Graz, Austria.
Joachim ReidlInstitute of Molecular Biosciences, University of Graz, NAWI Graz, BioTechMed-Graz, Humboldtstraße 50, A-8010 Graz, Austria.
Mario F FeldmanDepartment of Biological Sciences, University of Alberta, CW405 Biological Sciences Building, Edmonton, Alberta, Canada T6G 2E9.
Stefan SchildInstitute of Molecular Biosciences, University of Graz, NAWI Graz, BioTechMed-Graz, Humboldtstraße 50, A-8010 Graz, Austria.

Funding

Austrian Science Fund FWF P 25193Austrian Science Fund FWF P 25691CIHR
6 · The paper itself

Abstract

Bacterial outer membrane vesicles (OMVs) have important biological roles in pathogenesis and intercellular interactions, but a general mechanism of OMV formation is lacking. Here we show that the VacJ/Yrb ABC (ATP-binding cassette) transport system, a proposed phospholipid transporter, is involved in OMV formation. Deletion or repression of VacJ/Yrb increases OMV production in two distantly related Gram-negative bacteria, Haemophilus influenzae and Vibrio cholerae. Lipidome analyses demonstrate that OMVs from VacJ/Yrb-defective mutants in H. influenzae are enriched in phospholipids and certain fatty acids. Furthermore, we demonstrate that OMV production and regulation of the VacJ/Yrb ABC transport system respond to iron starvation. Our results suggest a new general mechanism of OMV biogenesis based on phospholipid accumulation in the outer leaflet of the outer membrane. This mechanism is highly conserved among Gram-negative bacteria, provides a means for regulation, can account for OMV formation under all growth conditions, and might have important pathophysiological roles in vivo.

Indexed as

Organelle BiogenesisAnimalsATP-Binding Cassette TransportersCytoplasmic VesiclesEscherichia coliFemaleHaemophilus influenzaeMice, Inbred BALB CVibrio choleraeATP-Binding Cassette Transporters

Identifiers

PMID26806181
PMCPMC4737802

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.