ArticleCell reports2016
Serum-Based Culture Conditions Provoke Gene Expression Variability in Mouse Embryonic Stem Cells as Revealed by Single-Cell Analysis.
Article in Cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Accurate and robust 3D genome feature discovery from multiplexed DNA FISH.bioRxiv : the preprint server for biology · 2025Article
- The impact of the embryonic DNA methylation program on CTCF-mediated genome regulation.Nucleic acids research · 2024Article
- Advances in induced pluripotent stem cell-derived cardiac myocytes: technological breakthroughs, key discoveries and new applications.The Journal of physiology · 2024Review
- scBoolSeq: Linking scRNA-seq statistics and Boolean dynamics.PLoS computational biology · 2024Article
- Esm-1 mediates transcriptional polarization associated with diabetic kidney disease.American journal of physiology. Renal physiology · 2024Article
- ARTseq-FISH reveals position-dependent differences in gene expression of micropatterned mESCs.Nature communications · 2024Article
- Noninvasive analysis of contractility during identical maturations revealed two phenotypes in ventricular but not in atrial iPSC-CM.American journal of physiology. Heart and circulatory physiology · 2024Article
- P53 and BCL-2 family proteins PUMA and NOXA define competitive fitness in pluripotent cell competition.PLoS genetics · 2024Article
- Characterization of a Distinct State in the Continuum of Pluripotency Facilitated by Inhibition of PKCζ in Mouse Embryonic Stem Cells.Stem cell reviews and reports · 2023Article
- Modularity and neuronal heterogeneity: Two properties that influenceFrontiers in cellular neuroscience · 2023Article
- Epigenetics as "conductor" in "orchestra" of pluripotent states.Cell and tissue research · 2022Review
- Serum-free cultures of C2C12 cells show different muscle phenotypes which can be estimated by metabolic profiling.Scientific reports · 2022Article
- From embryos to embryoids: How external signals and self-organization drive embryonic development.Stem cell reports · 2021Review
- Converging genetic and epigenetic drivers of paediatric acute lymphoblastic leukaemia identified by an information-theoretic analysis.Nature biomedical engineering · 2021Article
- hnRNPLL controls pluripotency exit of embryonic stem cells by modulating alternative splicing of Tbx3 and Bptf.The EMBO journal · 2021Article
- Organization of the Pluripotent Genome.Cold Spring Harbor perspectives in biology · 2021Review
- Cell Signaling Coordinates Global PRC2 Recruitment and Developmental Gene Expression in Murine Embryonic Stem Cells.iScience · 2020Article
- A Similar Speciation Process Relying on Cellular Stochasticity in Microbial and Cancer Cell Populations.iScience · 2020Review
- Leaf Trichome Distribution Pattern inPlants (Basel, Switzerland) · 2020Article
- Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells.Science advances · 2020Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Variation in gene expression is an important feature of mouse embryonic stem cells (ESCs). However, the mechanisms responsible for global gene expression variation in ESCs are not fully understood. We performed single-cell mRNA-seq analysis of mouse ESCs and uncovered significant heterogeneity in ESCs cultured in serum. We define highly variable gene clusters with distinct chromatin states and show that bivalent genes are prone to expression variation. At the same time, we identify an ESC-priming pathway that initiates the exit from the naive ESC state. Finally, we provide evidence that a large proportion of intracellular network variability is due to the extracellular culture environment. Serum-free culture reduces cellular heterogeneity and transcriptome variation in ESCs.
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Registered trials
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