ArticleCurrent microbiology2016
Application of Routine Diagnostic Procedure, VITEK 2 Compact, MALDI-TOF MS, and PCR Assays in Identification Procedure of Bacterial Strain with Ambiguous Phenotype.
Article in Current microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 33 citations in OpenAlex.
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- Antimicrobial Resistance, Pathogenic, and Molecular Characterization ofPathogens (Basel, Switzerland) · 2022Article
- Proteomics-based identification of orchid-associated bacteria colonizing theSaudi journal of biological sciences · 2021Article
- The Phylogenetic Structure of Reptile, Avian and UropathogenicInternational journal of molecular sciences · 2021Article
- Evaluation of the Autof MS1000 mass spectrometer in the identification of clinical isolates.BMC microbiology · 2020Article
- Pathogenic characteristics of sputum and bronchoalveolar lavage fluid samples from patients with lower respiratory tract infection in a large teaching hospital in China: a retrospective study.BMC pulmonary medicine · 2020Article
- The heat shock protein 70 gene as a new alternative molecular marker for the taxonomic identification of Streptomyces strains.AMB Express · 2018Article
- Identification of Yersinia enterocolitica isolates from humans, pigs and wild boars by MALDI TOF MS.BMC microbiology · 2018Article
- Microbial communities associated with the co-metabolism of free cyanide and thiocyanate under alkaline conditions.3 Biotech · 2018Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In diagnostic microbiology as well as in microbiological research, the identification of a microorganism is a crucial and decisive stage. A broad choice of methods is available, based on both phenotypic and molecular properties of microbes. The aim of this study was to compare the application of phenotypic and molecular tools in bacterial identification on the example of Gram-negative intestine rod with an ambiguous phenotype. Different methods of identification procedure, which based on various properties of bacteria, were applied, e.g., microscopic observation of single-bacterial cells, macroscopic observation of bacterial colonies morphology, the automated system of microorganism identification (biochemical tests), the mass spectrometry method (analysis of bacterial proteome), and genetic analysis with PCR reactions. The obtained results revealed discrepancies in the identification of the tested bacterial strain with an atypical phenotype: mucous morphology of colonies, not characteristic for either E. coli and Citrobacter spp., mass spectrometry analysis of proteome initially assigned the tested strain to Citrobacter genus (C. freundii) and biochemical profiles pointed to Escherichia coli. A decisive method in the current study was genetic analysis with PCR reactions which identified conserved genetic sequences highly specific to E. coli species in the genome of the tested strain.
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