ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2016
Digital antimicrobial susceptibility testing using the MilliDrop technology.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The trial behind it
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Who cites it
13 citing papers in PubMed.
- Low-Frequency Segmented-Flow Microfluidics for Biphasic Chemical Sensing.Sensors and actuators. B, Chemical · 2026Article
- The Potential Clinical Applications of a Microfluidic Lab-on-a-Chip for the Identification and Antibiotic Susceptibility Testing ofDentistry journal · 2023Review
- Under-oil open microfluidic systems for rapid phenotypic antimicrobial susceptibility testing.Lab on a chip · 2023Article
- The new microbiology: cultivating the future of microbiome-directed medicine.American journal of physiology. Gastrointestinal and liver physiology · 2020Review
- Review
- Droplet-Based Screening for the Investigation of Microbial Nonlinear Dose-Response Characteristics System, Background and Examples.Micromachines · 2020Article
- Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology.Micromachines · 2019Review
- Optimizing peptide nucleic acid probes for hybridization-based detection and identification of bacterial pathogens.The Analyst · 2019Article
- One System for All: Is Mass Spectrometry a Future Alternative for Conventional Antibiotic Susceptibility Testing?Frontiers in microbiology · 2019Review
- In vitro antimicrobial susceptibility testing methods: agar dilution to 3D tissue-engineered models.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Review
- Emerging Microtechnologies and Automated Systems for Rapid Bacterial Identification and Antibiotic Susceptibility Testing.SLAS technology · 2017Article
- Stochastically reduced communities-Microfluidic compartments as model and investigation tool for soil microorganism growth in structured spaces.Engineering in life sciences · 2017Article
- Review: Microbial analysis in dielectrophoretic microfluidic systems.Analytica chimica acta · 2017Review
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present the MilliDrop Analyzer (MDA), a droplet-based millifluidic system for digital antimicrobial susceptibility testing (D-AST), which enables us to determine minimum inhibitory concentrations (MICs) precisely and accurately. The MilliDrop technology was validated by using resazurin for fluorescence readout, for comparison with standard methodology, and for conducting reproducibility studies. In this first assessment, the susceptibility of a reference Gram-negative strain Escherichia coli ATCC 25922 to gentamicin, chloramphenicol, and nalidixic acid were tested by the MDA, VITEK®2, and broth microdilution as a reference standard. We measured the susceptibility of clinically relevant Gram-positive strains of Staphylococcus aureus to vancomycin, including vancomycin-intermediate S. aureus (VISA), heterogeneous vancomycin-intermediate S. aureus (hVISA), and vancomycin-susceptible S. aureus (VSSA) strains. The MDA provided results which were much more accurate than those of VITEK®2 and standard broth microdilution. The enhanced accuracy enabled us to reliably discriminate between VSSA and hVISA strains.
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Registered trials
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