ArticleJournal of industrial microbiology & biotechnology2016
Production of biorenewable styrene: utilization of biomass-derived sugars and insights into toxicity.
Article in Journal of industrial microbiology & biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Biomass valorization using 3D-printed catalysts.Communications chemistry · 2025Review
- Artesunate, EDTA, and colistin work synergistically against MCR-negative and -positive colistin-resistanteLife · 2025Article
- SIGHT-A System for Solvent-Tight Incubation and Growth Monitoring in High Throughput.Engineering in life sciences · 2025Article
- Microbial engineering for monocyclic aromatic compounds production.FEMS microbiology reviews · 2025Review
- Styrene Production in Genetically EngineeredBiotech (Basel (Switzerland)) · 2024Article
- Bioupgrading of the aqueous phase of pyrolysis oil from lignocellulosic biomass: a platform for renewable chemicals and fuels from the whole fraction of biomass.Bioresources and bioprocessing · 2023Review
- Polymers without Petrochemicals: Sustainable Routes to Conventional Monomers.Chemical reviews · 2023Review
- Characterizing Escherichia coli's transcriptional response to different styrene exposure modes reveals novel toxicity and tolerance insights.Journal of industrial microbiology & biotechnology · 2021Article
- Reverse engineering of fatty acid-tolerant Escherichia coli identifies design strategies for robust microbial cell factories.Metabolic engineering · 2020Article
- Streamlined assessment of membrane permeability and its application to membrane engineering of Escherichia coli for octanoic acid tolerance.Journal of industrial microbiology & biotechnology · 2019Article
- Enhanced production of styrene by engineered Escherichia coli and in situ product recovery (ISPR) with an organic solvent.Microbial cell factories · 2019Article
- Rational Engineering of Phenylalanine Accumulation inFrontiers in bioengineering and biotechnology · 2019Article
- Effect of n-Caproate Concentration on Chain Elongation and Competing Processes.ACS sustainable chemistry & engineering · 2018Article
- A systematic optimization of styrene biosynthesis inBiotechnology for biofuels · 2018Article
- Improving Escherichia coli membrane integrity and fatty acid production by expression tuning of FadL and OmpF.Microbial cell factories · 2017Article
- Engineering membrane and cell-wall programs for tolerance to toxic chemicals: Beyond solo genes.Current opinion in microbiology · 2016Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fermentative production of styrene from glucose has been previously demonstrated in Escherichia coli. Here, we demonstrate the production of styrene from the sugars derived from lignocellulosic biomass depolymerized by fast pyrolysis. A previously engineered styrene-producing strain was further engineered for utilization of the anhydrosugar levoglucosan via expression of levoglucosan kinase. The resulting strain produced 240 ± 3 mg L(-1) styrene from pure levoglucosan, similar to the 251 ± 3 mg L(-1) produced from glucose. When provided at a concentration of 5 g L(-1), pyrolytic sugars supported styrene production at titers similar to those from pure sugars, demonstrating the feasibility of producing this important industrial chemical from biomass-derived sugars. However, the toxicity of contaminant compounds in the biomass-derived sugars and styrene itself limit further gains in production. Styrene toxicity is generally believed to be due to membrane damage. Contrary to this prevailing wisdom, our quantitative assessment during challenge with up to 200 mg L(-1) of exogenously provided styrene showed little change in membrane integrity; membrane disruption was observed only during styrene production. Membrane fluidity was also quantified during styrene production, but no changes were observed relative to the non-producing control strain. This observation that styrene production is much more damaging to the membrane integrity than challenge with exogenously supplied styrene provides insight into the mechanism of styrene toxicity and emphasizes the importance of verifying proposed toxicity mechanisms during production instead of relying upon results obtained during exogenous challenge.
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