Evidence map›Paper›PMID 26802938›Full record

ArticleProstaglandins, leukotrienes, and essential fatty acids2016

Brown but not white adipose cells synthesize omega-3 docosahexaenoic acid in culture.

Xia Qin, Hui Gyu Park, Ji Yao Zhang, Peter Lawrence, Guowen Liu, Nivetha Subramanian, Kumar S D Kothapalli, J Thomas Brenna

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Prostaglandins, leukotrienes, and essential fatty acids, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.3field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Adaptive thermogenesis by dietary n-3 polyunsaturated fatty acids: Emerging evidence and mechanisms.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2019
    Review
  5. PPAR Agonists and Metabolic Syndrome: An Established Role?International journal of molecular sciences · 2018
    Review
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Xia QinDivision of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA; College of Veterinary Medicine, Jilin University, Changchun, Jilin 130062, China.
Hui Gyu ParkDivision of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA.
Ji Yao ZhangDivision of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA.
Peter LawrenceDivision of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA.
Guowen LiuDivision of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA; College of Veterinary Medicine, Jilin University, Changchun, Jilin 130062, China.
Nivetha SubramanianDivision of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA.
Kumar S D KothapalliDivision of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA.
J Thomas BrennaDivision of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA.
Cornell University · USJilin University · CN

Funding

Molecular mechanism of omega-3 responseR01AT007003 · NCCIH · UNIVERSITY OF TEXAS AT AUSTIN · PI BRENNA, JAMES T. · 2013 to 2017
$1.9M
NCCIH NIH HHS R01 AT007003NCCIH NIH HHS R01AT007003
6 · The paper itself

Abstract

Adipose tissue is a complex endocrine organ which coordinates several crucial biological functions including fatty acid metabolism, glucose metabolism, energy homeostasis, and immune function. Brown adipose tissue (BAT) is most abundant in young infants during the brain growth spurt when demands for omega-3 docosahexaenoic acid (DHA, 22:6n-3) is greatest for brain structure. Our aim was to characterize relative biosynthesis of omega-3 long chain polyunsaturated fatty acids (LCPUFA) from precursors in cultured white (WAT) and brown (BAT) cells and study relevant gene expression. Mouse WAT and BAT cells were grown in regular DMEM media to confluence, and differentiation was induced. At days 0 and 8 cells were treated with albumin bound d5-18:3n-3 (d5-ALA) and analyzed 24h later. d5-ALA increased cellular eicosapentaenoic acid (EPA, 20:5n-3) and docosapentaenoic acid (DPA, 22:5n-3) in undifferentiated BAT cells, whereas differentiated BAT cells accumulated 20:4n-3, EPA and DPA. DHA as a fraction of total omega-3 LCPUFA was greatest in differentiated BAT cells compared to undifferentiated cells. Undifferentiated WAT cells accumulated EPA, whereas differentiated cells accumulated DPA. WAT accumulated trace newly synthesized DHA. Zic1 a classical brown marker and Prdm16 a key driver of brown fat cell fate are expressed only in BAT cells. Ppargc1a is 15 fold higher in differentiated BAT cells. We conclude that in differentiated adipose cells accumulating fat, BAT cells but not WAT cells synthesize DHA, supporting the hypothesis that BAT is a net producer of DHA.

Indexed as

Adipose TissueAdipose Tissue, BrownAdipose Tissue, WhiteAnimalsCell DifferentiationCell LineDocosahexaenoic AcidsEicosapentaenoic AcidFatty Acids, Omega-3Fatty Acids, UnsaturatedMiceDocosahexaenoic Acidsdocosapentaenoic acidEicosapentaenoic AcidFatty Acids, Omega-3Fatty Acids, UnsaturatedBrown adipose tissueDifferentiatedOmega-3 long chain polyunsaturated fatty acidsUndifferentiatedWhite adipose tissue

Identifiers

PMID26802938
PMCPMC4724391
OpenAlexW2185047662

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.