ArticleMolecular biology and evolution2016
Purifying Selection on Exonic Splice Enhancers in Intronless Genes.
Article in Molecular biology and evolution, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Exploring Disordered Regions of Human Spliceosome Proteins.The journal of physical chemistry letters · 2026Article
- Identification and characterization of the DREB family in Chinese yam and functional study of DoDREB28 under drought stress.BMC plant biology · 2026Article
- Tumor-promoting effect and tumor immunity of SRSFs.Frontiers in cell and developmental biology · 2025Review
- Selection on synonymous sites: the unwanted transcript hypothesis.Nature reviews. Genetics · 2024Review
- A novel HPV16 splicing enhancer critical for viral oncogene expression and cell immortalization.Nucleic acids research · 2024Article
- SRSF2 is required for mRNA splicing during spermatogenesis.BMC biology · 2023Article
- Trends in the evolution of intronless genes inFrontiers in plant science · 2023Article
- SRSF1-mediated alternative splicing is required for spermatogenesis.International journal of biological sciences · 2023Article
- Review
- Genome-wide analysis of European sea bass provides insights into the evolution and functions of single-exon genes.Ecology and evolution · 2021Article
- Codon Usage and Splicing Jointly Influence mRNA Localization.Cell systems · 2020Article
- A Depletion of Stop Codons in lincRNA is Owing to Transfer of Selective Constraint from Coding Sequences.Molecular biology and evolution · 2020Article
- Serine/arginine-rich splicing factors: the bridge linking alternative splicing and cancer.International journal of biological sciences · 2020Review
- Intronless mRNAs transit through nuclear speckles to gain export competence.The Journal of cell biology · 2018Article
- Exonic splice regulation imposes strong selection at synonymous sites.Genome research · 2018Article
- Depletion of somatic mutations in splicing-associated sequences in cancer genomes.Genome biology · 2017Article
- Estimating the prevalence of functional exonic splice regulatory information.Human genetics · 2017Review
- Both Maintenance and Avoidance of RNA-Binding Protein Interactions Constrain Coding Sequence Evolution.Molecular biology and evolution · 2017Article
- Engineering of PEDF-Expressing Primary Pigment Epithelial Cells by the SB Transposon System Delivered by pFAR4 Plasmids.Molecular therapy. Nucleic acids · 2017Article
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2 authors.
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Abstract
Exonic splice enhancers (ESEs) are short nucleotide motifs, enriched near exon ends, that enhance the recognition of the splice site and thus promote splicing. Are intronless genes under selection to avoid these motifs so as not to attract the splicing machinery to an mRNA that should not be spliced, thereby preventing the production of an aberrant transcript? Consistent with this possibility, we find that ESEs in putative recent retrocopies are at a higher density and evolving faster than those in other intronless genes, suggesting that they are being lost. Moreover, intronless genes are less dense in putative ESEs than intron-containing ones. However, this latter difference is likely due to the skewed base composition of intronless sequences, a skew that is in line with the general GC richness of few exon genes. Indeed, after controlling for such biases, we find that both intronless and intron-containing genes are denser in ESEs than expected by chance. Importantly, nucleotide-controlled analysis of evolutionary rates at synonymous sites in ESEs indicates that the ESEs in intronless genes are under purifying selection in both human and mouse. We conclude that on the loss of introns, some but not all, ESE motifs are lost, the remainder having functions beyond a role in splice promotion. These results have implications for the design of intronless transgenes and for understanding the causes of selection on synonymous sites.
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