ArticleBMC infectious diseases2016
Characterization of Sarcoptes scabiei cofilin gene and assessment of recombinant cofilin protein as an antigen in indirect-ELISA for diagnosis.
Article in BMC infectious diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Scabies as a Neglected Tropical Disease: A Comprehensive Review of Pathogenesis, Epidemiology, Clinical Manifestations, Diagnosis and Treatment.The Yale journal of biology and medicine · 2025Review
- Novel SARS-COV2 poly epitope phage-based candidate vaccine and its immunogenicity.Research in pharmaceutical sciences · 2024Article
- Laboratory-based diagnosis of scabies: a review of the current status.Transactions of the Royal Society of Tropical Medicine and Hygiene · 2022Review
- Comparative analysis of the allergenic characteristics and serodiagnostic potential of recombinant chitinase-like protein-5 and -12 from Sarcoptes scabiei.Parasites & vectors · 2021Article
- Molecular characterization and serodiagnostic potential of two serpin proteins in Psoroptes ovis var. cuniculi.Parasites & vectors · 2020Article
- Characterization of mitochondrial COX-1 gene of Sarcoptes scabiei from rabbits in East Java, Indonesia.Journal of advanced veterinary and animal research · 2019Article
- Sequence analysis of the cytochrome c oxidase subunit 1 gene ofVeterinary world · 2019Article
- Serodiagnostic Potential of Alpha-Enolase FromFrontiers in microbiology · 2018Article
- Molecular characteristics and serodiagnostic potential of chitinase-like protein fromOncotarget · 2017Article
- Characterization ofThe American journal of tropical medicine and hygiene · 2017Article
- Expression and characterisation of a Sarcoptes scabiei protein tyrosine kinase as a potential antigen for scabies diagnosis.Scientific reports · 2017Article
- A review of Sarcoptes scabiei: past, present and future.Parasites & vectors · 2017Review
- Article
- Scabies: Advances in Noninvasive Diagnosis.PLoS neglected tropical diseases · 2016Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundScabies impairs the health of humans and animals and causes heavy economic losses. Traditional diagnostic methods for scabies are inefficient and ineffective, and so far there is no commercial immunodiagnostic or molecular based test for scabies.
methodsHere, we used recombinant Sarcoptes scabiei cofilin protein as an antigen to establish indirect ELISA. S. scabiei cofilin is highly homologous to Dermatophagoides farinae Der f 31 allergen (90% identity). The S. scabiei cofilin gene was cloned and expressed in Escherichia coli to obtain recombinant protein. Western blotting and fluorescence immunohistochemistry were carried out, and we established an indirect ELISA method and detected 33 serum samples from scabies infected rabbits and 30 serum samples from naïve rabbits.
resultsWestern blotting demonstrated that S. scabiei cofilin possessed good immunogenicity and fluorescence immunohistochemistry showed the S. scabiei cofilin is widespread in the splanchnic area of mites. In ELISA, a cut-off value of 0.188 was determined to judge experimental positive and negative serum values. Specificity and sensitivity of the ELISA were 87.9 and 83.33%, respectively.
conclusionsRecombinant S. scabiei cofilin showed potential value as a diagnostic antigen. The ELISA method established could be used in clinical diagnosis and provide experimental information in minimal or asymptomatic infection.
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