ArticleApplied and environmental microbiology2016
Improvement of Biological Indicators by Uniformly Distributing Bacillus subtilis Spores in Monolayers To Evaluate Enhanced Spore Decontamination Technologies.
Article in Applied and environmental microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed.
- Development of a functional and disinfectable sewage vent high-efficiency particulate air filter system and its validation using vaporized hydrogen peroxide.Applied and environmental microbiology · 2026Article
- Attention-driven UNet enhancement for accurate segmentation of bacterial spore outgrowth in microscopy images.Scientific reports · 2025Article
- Modified protocol comparing sporicidal activity of different non-thermal plasma generating devices.Scientific reports · 2025Article
- Evaluating the impact of ultraviolet C exposure conditions on coliphage MS2 inactivation on surfaces.Letters in applied microbiology · 2022Article
- Characterization of a robot-assisted UV-C disinfection for the inactivation of surface-associated microorganisms and viruses.Journal of photochemistry and photobiology · 2022Article
- Overcoming challenges due to enhanced biomedical waste generation during COVID-19 pandemic.The Science of the total environment · 2022Review
- Inactivation of Bacillus anthracis and Bacillus atrophaeus spores on different surfaces with ultraviolet light produced with a low-pressure mercury vapor lamp or light emitting diodes.Journal of applied microbiology · 2021Article
- Airborne Disinfection by Dry Fogging Efficiently Inactivates Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), Mycobacteria, and Bacterial Spores and Shows Limitations of Commercial Spore Carriers.Applied and environmental microbiology · 2021Article
- Critical Capability Needs for Reduction of Transmission of SARS-CoV-2 Indoors.Frontiers in bioengineering and biotechnology · 2021Review
- Review of Decontamination Techniques for the Inactivation of Bacillus anthracis and Other Spore-Forming Bacteria Associated with Building or Outdoor Materials.Environmental science & technology · 2019Review
- Novel 'Bacteriospray' Method Facilitates the Functional Screening of Metagenomic Libraries for Antimicrobial Activity.Methods and protocols · 2019Article
- Experimental studies addressing the longevity of Bacillus subtilis spores - The first data from a 500-year experiment.PloS one · 2018Article
- Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy.Journal of visualized experiments : JoVE · 2017Article
- Effects of Low-Temperature Plasma-Sterilization on Mars Analog Soil Samples Mixed with Deinococcus radiodurans.Life (Basel, Switzerland) · 2016Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Novel decontamination technologies, including cold low-pressure plasma and blue light (400 nm), are promising alternatives to conventional surface decontamination methods. However, the standardization of the assessment of such sterilization processes remains to be accomplished. Bacterial endospores of the genera Bacillus and Geobacillus are frequently used as biological indicators (BIs) of sterility. Ensuring standardized and reproducible BIs for reliable testing procedures is a significant problem in industrial settings. In this study, an electrically driven spray deposition device was developed, allowing fast, reproducible, and homogeneous preparation of Bacillus subtilis 168 spore monolayers on glass surfaces. A detailed description of the structural design as well as the operating principle of the spraying device is given. The reproducible formation of spore monolayers of up to 5 × 10(7) spores per sample was verified by scanning electron microscopy. Surface inactivation studies revealed that monolayered spores were inactivated by UV-C (254 nm), low-pressure argon plasma (500 W, 10 Pa, 100 standard cubic cm per min), and blue light (400 nm) significantly faster than multilayered spores were. We have thus succeeded in the uniform preparation of reproducible, highly concentrated spore monolayers with the potential to generate BIs for a variety of nonpenetrating surface decontamination techniques.
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