ArticlePlant biotechnology journal2016
Expression of ZmGA20ox cDNA alters plant morphology and increases biomass production of switchgrass (Panicum virgatum L.).
Article in Plant biotechnology journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 65 citations in OpenAlex.
- Genetically Modified Plants in Agriculture.Biology · 2026Review
- Dissecting the genetic basis of agronomic traits by multi-trait GWAS and genetic networks in maize (aBIOTECH · 2025Article
- Blocking miR528 function promotes tillering and regrowth in switchgrass.Plant biotechnology journal · 2024Article
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- Citrus ACC synthase CiACS4 regulates plant height by inhibiting gibberellin biosynthesis.Plant physiology · 2023Article
- Rate of crop-weed hybridization inEvolutionary applications · 2023Article
- Differential roles ofFrontiers in plant science · 2023Article
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- The distribution of bioactive gibberellins along peach annual shoots is closely associated with PpGA20ox and PpGA2ox expression profiles.BMC genomics · 2022Article
- Enhancing plant growth and biomass production by overexpression of GA20ox gene under control of a root preferential promoter.Transgenic research · 2022Article
- Genetic Determinants of Biomass in CFrontiers in plant science · 2022Review
- Overexpression of PvWOX3a in switchgrass promotes stem development and increases plant height.Horticulture research · 2021Article
- MiR396-GRF module associates with switchgrass biomass yield and feedstock quality.Plant biotechnology journal · 2021Article
- Gibberellin Oxidase Gene Family inInternational journal of molecular sciences · 2021Article
- Crosstalk between auxin and gibberellin during stalk elongation in flowering Chinese cabbage.Scientific reports · 2021Article
- Expression of a bacterial 3-dehydroshikimate dehydratase (QsuB) reduces lignin and improves biomass saccharification efficiency in switchgrass (Panicum virgatum L.).BMC plant biology · 2021Article
- Overexpression ofFrontiers in plant science · 2021Article
- Genome-wide analysis of gibberellin-dioxygenases gene family and their responses to GA applications in maize.PloS one · 2021Article
- Systematic Analysis of Gibberellin Pathway Components inInternational journal of molecular sciences · 2020Article
- Heteroexpression ofBiotechnology for biofuels · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Switchgrass (Panicum virgatum L.) is considered a model herbaceous energy crop for the USA, for its adaptation to marginal land, low rainfall and nutrient-deficient soils; however, its low biomass yield is one of several constraints, and this might be rectified by modulating plant growth regulator levels. In this study, we have determined whether the expression of the Zea mays gibberellin 20-oxidase (ZmGA20ox) cDNA in switchgrass will improve biomass production. The ZmGA20ox gene was placed under the control of constitutive CaMV35S promoter with a strong TMV omega enhancer, and introduced into switchgrass via Agrobacterium-mediated transformation. The transgene integration and expression levels of ZmGA20ox in T0 plants were analysed using Southern blot and qRT-PCR. Under glasshouse conditions, selected transgenic plants exhibited longer leaves, internodes and tillers, which resulted in twofold increased biomass. These phenotypic alterations correlated with the levels of transgene expression and the particular gibberellin content. Expression of ZmGA20ox also affected the expression of genes coding for key enzymes in lignin biosynthesis. Our results suggest that the employment of ectopic ZmGA20ox and selection for natural variants with high level expression of endogenous GA20ox are appropriate approaches to increase biomass production of switchgrass and other monocot biofuel crops.
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