ArticlePlant biotechnology journal2016
Overexpression of MdbHLH104 gene enhances the tolerance to iron deficiency in apple.
Article in Plant biotechnology journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 130 citations in OpenAlex.
- Functional Analysis ofCurrent issues in molecular biology · 2025Article
- Genome-wide identification and analysis of the apple HBMC plant biology · 2025Article
- Genome-Wide Identification of the bHLH Gene Family inInternational journal of molecular sciences · 2024Article
- Functions and Regulatory Mechanisms of bHLH Transcription Factors during the Responses to Biotic and Abiotic Stresses in Woody Plants.Plants (Basel, Switzerland) · 2024Review
- Functional Identification of MhPYL4 Involved in Iron-Deficiency Stress in Malus Halliana Koehne.Plants (Basel, Switzerland) · 2024Article
- Calmodulin-like protein MdCML15 interacts with MdBT2 to modulate iron homeostasis in apple.Horticulture research · 2024Article
- 5-Aminolevulinic acid improves cold resistance through regulation of SlMYB4/SlMYB88-SlGSTU43 module to scavenge reactive oxygen species in tomato.Horticulture research · 2024Article
- Overexpression of PavbHLH28 from Prunus avium enhances tolerance to cold stress in transgenic Arabidopsis.BMC plant biology · 2023Article
- AcbHLH144 transcription factor negatively regulates phenolic biosynthesis to modulate pineapple internal browning.Horticulture research · 2023Article
- Article
- Genome-Wide Identification and Analysis of bHLH Transcription Factors Related to Anthocyanin Biosynthesis inInternational journal of molecular sciences · 2023Article
- Genome-wide identification of bHLH transcription factors and their response to salt stress inFrontiers in plant science · 2023Article
- Molecular cloning and functional characterization of MhHEC2-like genes in Malus halliana reveals it enhances Fe (iron) deficiency tolerance.Functional & integrative genomics · 2022Article
- Brassinolide alleviates Fe deficiency-induced stress by regulating the Fe absorption mechanism in Malus hupehensis Rehd.Plant cell reports · 2022Article
- Root Breeding in the Post-Genomics Era: From Concept to Practice in Apple.Plants (Basel, Switzerland) · 2022Review
- Long-distance mobile mRNA CAX3 modulates iron uptake and zinc compartmentalization.EMBO reports · 2022Article
- Brassinolide improves the tolerance ofFrontiers in plant science · 2022Article
- MYB308-mediated transcriptional activation of plasma membrane HHorticulture research · 2022Article
- Genome-wide association study uncovers major genetic loci associated with seed flooding tolerance in soybean.BMC plant biology · 2021Article
- Mechanisms and regulation of organic acid accumulation in plant vacuoles.Horticulture research · 2021Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fe deficiency is a widespread nutritional disorder in plants. The basic helix-loop-helix (bHLH) transcription factors (TFs), especially Ib subgroup bHLH TFs which are involved in iron uptake, have been identified. In this study, an IVc subgroup bHLH TF MdbHLH104 was identified and characterized as a key component in the response to Fe deficiency in apple. The overexpression of the MdbHLH104 gene noticeably increased the H(+) -ATPase activity under iron limitation conditions and the tolerance to Fe deficiency in transgenic apple plants and calli. Further investigation showed that MdbHLH104 proteins bonded directly to the promoter of the MdAHA8 gene, thereby positively regulating its expression, the plasma membrane (PM) H(+) -ATPase activity and Fe uptake. Similarly, MdbHLH104 directly modulated the expression of three Fe-responsive bHLH genes, MdbHLH38, MdbHLH39 and MdPYE. In addition, MdbHLH104 interacted with 5 other IVc subgroup bHLH proteins to coregulate the expression of the MdAHA8 gene, the activity of PM H(+) -ATPase and the content of Fe in apple calli. Therefore, MdbHLH104 acts together with other apple bHLH TFs to regulate Fe uptake by modulating the expression of the MdAHA8 gene and the activity of PM H(+) -ATPase in apple.
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