ArticleBMC genomics2016
Suboptimal culture conditions induce more deviations in gene expression in male than female bovine blastocysts.
Article in BMC genomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- EngineeringBiomolecules · 2026Article
- Epitranscriptomic modifications in embryonic development: insights into natural and ART-induced mechanisms and implications.Human reproduction update · 2026Review
- Sexually dimorphic gene expression responses of bovine embryos to the maternal microenvironment on day 13 of gestation.BMC genomics · 2025Article
- Emerging Contributions of Pluripotent Stem Cells to Reproductive Technologies in Veterinary Medicine.Journal of developmental biology · 2024Review
- Evaluating cell culture reliability in pediatric brain tumor primary cells through DNA methylation profiling.NPJ precision oncology · 2024Article
- Sexual dimorphic miRNA-mediated response of bovine elongated embryos to the maternal microenvironment.PloS one · 2024Article
- Effect of lipotoxicity on mitochondrial function and epigenetic programming during bovine in vitro embryo production.Scientific reports · 2023Article
- Advancing bovineFrontiers in bioengineering and biotechnology · 2023Article
- Extracellular vesicles from oviductal and uterine fluids supplementation in sequential in vitro culture improves bovine embryo quality.Journal of animal science and biotechnology · 2022Article
- Non-Invasive Identification of Sex in Cultured Bovine Embryos by UHPLC-MS/MS Metabolomics.Metabolomics : Official journal of the Metabolomic Society · 2022Article
- In vitro production of desired sex ovine embryos modulating polarity of oocytes for sex-specific sperm binding during fertilization.Scientific reports · 2022Article
- Lycopene Supplementation to Serum-Free Maturation Medium Improves In Vitro Bovine Embryo Development and Quality and Modulates Embryonic Transcriptomic Profile.Antioxidants (Basel, Switzerland) · 2022Article
- Fatty Acid Supplementation DuringFrontiers in cell and developmental biology · 2022Article
- Epigenomic and transcriptomic analyses reveal early activation of the HPG axis in in vitro-produced male dairy calves.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Culture Medium and Sex Drive Epigenetic Reprogramming in Preimplantation Bovine Embryos.International journal of molecular sciences · 2021Article
- Transcriptome of D14 in vivo x in vitro bovine embryos: is there any difference?In vitro cellular & developmental biology. Animal · 2021Article
- Regulation of gene expression in the bovine blastocyst by colony-stimulating factor 2 is disrupted by CRISPR/Cas9-mediated deletion of CSF2RA.Biology of reproduction · 2021Article
- An improved method for specific-target preamplification PCR analysis of single blastocysts useful for embryo sexing and high-throughput gene expression analysis.Journal of dairy science · 2021Article
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12 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundSince the development of in vitro embryo production in cattle, different supplements have been added to culture media to support embryo development, with serum being the most popular. However, the addition of serum during embryo culture can induce high birthweights and low viability in calves (Large Offspring Syndrome). Analysis of global gene expression in bovine embryos produced under different conditions can provide valuable information to optimize culture media for in vitro embryo production.
resultsWe used RNA sequencing to examine the effect of in vitro embryo production, in either serum-containing or serum-free media, on the global gene expression pattern of individual bovine blastocysts. Compared to in vivo derived embryos, embryos produced in serum-containing medium had five times more differentially expressed genes than embryos produced in serum-free conditions (1109 vs. 207). Importantly, in vitro production in the presence of serum appeared to have a different impact on the embryos according to their sex, with male embryos having three times more genes differentially expressed than their female counterparts (1283 vs. 456). On the contrary, male and female embryos produced in serum-free conditions showed the same number (191 vs. 192) of genes expressed differentially; however, only 44 of those genes were common in both comparisons. The pathways affected by in vitro production differed depending on the type of supplementation. For example, embryos produced in serum-containing conditions had a lower expression of genes related to metabolism while embryos produced in serum-free conditions showed aberrations in genes involved in lipid metabolism.
conclusionsSerum supplementation had a major impact on the gene expression pattern of embryos, with male embryos being the most affected. The transcriptome of embryos produced in serum-free conditions showed a greater resemblance to that of in vivo derived embryos, although genes involved in lipid metabolism were altered. Male embryos appeared to be most affected by suboptimal in vitro culture, i.e. in the presence of serum.
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