ArticleExperimental & applied acarology2016
Immunohistochemistry and real-time PCR as diagnostic tools for detection of Borrelia burgdorferi sensu lato in ticks collected from humans.
Article in Experimental & applied acarology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Zoonotic Bacteria and Vector-Borne Protozoa in Troglophilus Bat Colonies in Sicily (Southern Italy): A Biomolecular Survey.Animals : an open access journal from MDPI · 2025Article
- Molecular Detection ofAnimals : an open access journal from MDPI · 2024Article
- Ticks and Tick-Borne Pathogens: Occurrence and Host Associations over Four Years of Wildlife Surveillance in the Liguria Region (Northwest Italy).Animals : an open access journal from MDPI · 2024Article
- A Canine Leptospirosis Clinical Case Due toVeterinary sciences · 2023Article
- Multiple Tick-Borne Pathogens inPathogens (Basel, Switzerland) · 2020Article
- Borrelia spp. in small mammals in Romania.Parasites & vectors · 2019Article
- Co-infection of Ticks: The Rule Rather Than the Exception.PLoS neglected tropical diseases · 2016Article
Corrections and comments
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Authors and funding
13 authors.
Funding
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Abstract
The objective of this study was to evaluate different methods used for detection of Borrelia burgdorferi sensu lato (s.l.) in ticks: immunohistochemistry followed by focus floating microscopy (FFM) and real-time polymerase chain reaction (real-time PCR) targeting the ospA and hbb genes. Additionally, an optimized ospA real-time PCR assay was developed with an integrated internal amplification control (IAC) for the detection of inhibition in the PCR assay and was validated as an improved screening tool for B. burgdorferi. One hundred and thirty-six ticks collected from humans in a hospital from Cluj-Napoca, Romania, were investigated regarding genus, stage of development and sex, and then tested by all three assays. A poor quality of agreement was found between FFM and each of the two real-time PCR assays, as assessed by concordance analysis (Cohen's kappa), whereas the agreement between the two real-time PCR assays was moderate. The present study argues for a low sensitivity of FFM and underlines that discordant results of different assays used for detection of B. burgdorferi in ticks are frequent.
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