ArticlePlant biotechnology journal2016
Multiplex sequencing of bacterial artificial chromosomes for assembling complex plant genomes.
Article in Plant biotechnology journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed.
- Differentiation of European yellow rust subraces within the 'Warrior(-)' genetic group.PloS one · 2025Article
- Are cereal grasses a single genetic system?Nature plants · 2024Article
- Natural variations of HvSRN1 modulate the spike rachis node number in barley.Plant communications · 2024Article
- TRITEX: chromosome-scale sequence assembly of Triticeae genomes with open-source tools.Genome biology · 2019Article
- Leaf Variegation and Impaired Chloroplast Development Caused by a Truncated CCT Domain Gene inThe Plant cell · 2019Article
- Prospects of pan-genomics in barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Review
- Genomic approaches for studying crop evolution.Genome biology · 2018Review
- Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum.PloS one · 2018Article
- Construction of a map-based reference genome sequence for barley, Hordeum vulgare L.Scientific data · 2017Article
- Article
- A Homolog of Blade-On-Petiole 1 and 2 (BOP1/2) Controls Internode Length and Homeotic Changes of the Barley Inflorescence.Plant physiology · 2016Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hierarchical shotgun sequencing remains the method of choice for assembling high-quality reference sequences of complex plant genomes. The efficient exploitation of current high-throughput technologies and powerful computational facilities for large-insert clone sequencing necessitates the sequencing and assembly of a large number of clones in parallel. We developed a multiplexed pipeline for shotgun sequencing and assembling individual bacterial artificial chromosomes (BACs) using the Illumina sequencing platform. We illustrate our approach by sequencing 668 barley BACs (Hordeum vulgare L.) in a single Illumina HiSeq 2000 lane. Using a newly designed parallelized computational pipeline, we obtained sequence assemblies of individual BACs that consist, on average, of eight sequence scaffolds and represent >98% of the genomic inserts. Our BAC assemblies are clearly superior to a whole-genome shotgun assembly regarding contiguity, completeness and the representation of the gene space. Our methods may be employed to rapidly obtain high-quality assemblies of a large number of clones to assemble map-based reference sequences of plant and animal species with complex genomes by sequencing along a minimum tiling path.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.