ArticlePlant biotechnology journal2016
Expression of a constitutively active nitrate reductase variant in tobacco reduces tobacco-specific nitrosamine accumulation in cured leaves and cigarette smoke.
Article in Plant biotechnology journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Metabolic engineering for tobacco quality improvement: Advances and perspectives.Metabolic engineering communications · 2026Review
- Involvement of NtInvVR1 in the increase of reducing sugars during the curing of virginia tobacco.Scientific reports · 2026Article
- Foliar application of magnesium and amino acids to off-season maize: a strategy to improve photosynthetic and antioxidant metabolism.Frontiers in plant science · 2026Article
- Expression of a constitutively active nitrate reductase increases SARS-CoV-2 Spike protein production in Nicotiana benthamiana leaves that otherwise show traits of senescence.Plant biotechnology journal · 2026Article
- Nitrate assimilation pathway is impacted in young tobacco plants overexpressing a constitutively active nitrate reductase or displaying a defective CLCNt2.BMC plant biology · 2024Article
- Integrative physiological and transcriptome analysis unravels the mechanism of low nitrogen use efficiency in burley tobacco.Plant direct · 2024Article
- Genetic regulation and manipulation of nicotine biosynthesis in tobacco: strategies to eliminate addictive alkaloids.Journal of experimental botany · 2024Review
- Reducing nitrate and tobacco-specific nitrosamine level in burley tobacco leaves through grafting on flue-cured tobacco rootstock.Plant direct · 2023Article
- Tobacco Nitrate and Free Radical Levels in the Mainstream Smoke of US Cigarette Brands.Chemical research in toxicology · 2023Article
- Effects of tobacco nitrate content on free radical levels in mainstream smoke.Free radical biology & medicine · 2022Article
- CLCNt2 Mediates Nitrate Content in Tobacco Leaf, Impacting the Production of Tobacco-Specific Nitrosamines in Cured Leaves.Frontiers in plant science · 2022Article
- Review
- Constitutive activation of nitrate reductase in tobacco alters flowering time and plant biomass.Scientific reports · 2021Article
- Combined Transcriptome and Proteome Analysis of Masson Pine (International journal of molecular sciences · 2020Article
- 24-Epibrassinolide promotes NOBMC plant biology · 2019Article
Corrections and comments
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Authors and funding
9 authors.
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Abstract
Burley tobaccos (Nicotiana tabacum) display a nitrogen-use-deficiency phenotype that is associated with the accumulation of high levels of nitrate within the leaf, a trait correlated with production of a class of compounds referred to as tobacco-specific nitrosamines (TSNAs). Two TSNA species, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and N-nitrosonornicotine (NNN), have been shown to be strong carcinogens in numerous animal studies. We investigated the potential of molecular genetic strategies to lower nitrate levels in burley tobaccos by overexpressing genes encoding key enzymes of the nitrogen-assimilation pathway. Of the various constructs tested, only the expression of a constitutively active nitrate reductase (NR) dramatically decreased free nitrate levels in the leaves. Field-grown tobacco plants expressing this NR variant exhibited greatly reduced levels of TSNAs in both cured leaves and mainstream smoke of cigarettes made from these materials. Decreasing leaf nitrate levels via expression of a constitutively active NR enzyme represents an exceptionally promising means for reducing the production of NNN and NNK, two of the most well-documented animal carcinogens found in tobacco products.
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