Evidence map›Paper›PMID 26799257›Full record

ArticleJournal of surgical oncology2016

Molecular targeting of papillary thyroid carcinoma with fluorescently labeled ratiometric activatable cell penetrating peptides in a transgenic murine model.

Ryan K Orosco, Elamprakash N Savariar, Philip A Weissbrod, Julio A Diaz-Perez, Michael Bouvet, Roger Y Tsien, Quyen T Nguyen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of surgical oncology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 16 citations in OpenAlex.

  1. Review
  2. Article
  3. Preclinical Imaging for the Study of Mouse Models of Thyroid Cancer.International journal of molecular sciences · 2017
    Review
  4. Article
  5. Advances in fluorescent-image guided surgery.Annals of translational medicine · 2016
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Ryan K OroscoDepartment of Surgery, Division of Head and Neck Surgery, University of California San Diego, San Diego, California.
Elamprakash N SavariarDepartment of Pharmacology, University of California San Diego, San Diego, California.
Philip A WeissbrodDepartment of Surgery, Division of Head and Neck Surgery, University of California San Diego, San Diego, California.
Julio A Diaz-PerezDepartment of Pathology, Moores Cancer Center, University of California San Diego, La Jolla, California.
Michael BouvetDepartment of Surgery, Division of Surgical Oncology, University of California San Diego, San Diego, California.
Roger Y TsienDepartment of Pharmacology, University of California San Diego, San Diego, California.
Quyen T NguyenDepartment of Surgery, Division of Head and Neck Surgery, University of California San Diego, San Diego, California.
University of San Diego · USUniversity of California, San Diego · US

Funding

DEVELOPMENT OF CEREBRAL SPECIALIZATIONSR01DC000128 · NIDCD · UNIVERSITY OF OREGON · PI NEVILLE, HELEN JUNE · 1989 to 2011
$6.0M
Injectable reporters to image tumors and guide resectionR01CA158448 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI NGUYEN, QUYEN · 2011 to 2015
$3.1M
Testing Fluorescently Labeled Probes for Nerve Imaging during SurgeryR01EB014929 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI NGUYEN, QUYEN · 2012 to 2016
$1.7M
LANGUAGE-BASED MEMORY AND SPATIAL MEMORY IN THE DEAFF32DC000128 · NIDCD · SALK INSTITUTE FOR BIOLOGICAL STUDIES · PI WILSON, MARGARET L · 1993 to 1996
–
NCI NIH HHS 1R01CA154448-01A1NCI NIH HHS R01 CA158448NIBIB NIH HHS R01 EB014929NIDCD NIH HHS F32 DC000128NIDCD NIH HHS R01 DC000128NIDCD NIH HHS T32-DC 000128Wellcome Trust
6 · The paper itself

Abstract

BACKGROUND AND

objectivesMolecularly targeted fluorescent molecules may help detect tumors that are unseen by traditional white-light surgical techniques. We sought to evaluate a fluorescent ratiometric activatable cell penetrating peptide (RACPP) for tumor detection in a transgenic model of PTC.

methodsThirteen BRAFV600E mice with PTC were studied-seven injected intravenously with RACPP, four controls with saline. Total thyroidectomy was performed with microscopic white-light visualization. Fluorescent imaging of post-thyroidectomy fields was performed, and tissue with increased signal was removed and evaluated for PTC. Final samples were analyzed by a pathologist blinded to conditions. Vocal cord function was evaluated postoperatively with video laryngoscopy.

resultsThe average in situ ratiometric (Cy5/Cy7) thyroid tumor-to-background contrast ratio was 2.27 +/- 0.91. Fluorescence-guided clean-up following thyroidectomy identified additional tumor in 2 of 7 RACPP animals (smallest dimension 1.2 mm), and decreased the number of animals with residual tumor from 4 to 3. All retained tumor foci on final pathology were smaller than 0.76 mm. Intact vocal abduction was present in all of the RACPP animals.

conclusionsRACPPs successfully targeted PTC in a transgenic thyroidectomy model, and allowed for residual tumor detection that reduced positive margins beyond what was possible with white-light surgery alone.

Indexed as

Cell-Penetrating PeptidesThyroidectomyAnimalsAnimals, Genetically ModifiedCarcinomaCarcinoma, PapillaryDisease Models, AnimalFluorescenceGlutamic AcidLaryngoscopyLarynxMiceMolecular Targeted TherapyProto-Oncogene Proteins B-rafStaining and LabelingThyroid Cancer, PapillaryBraf protein, mouseCell-Penetrating PeptidesGlutamic AcidProto-Oncogene Proteins B-rafValineactivatable cell penetrating peptideBRAF V600Elaryngoscopymurine modelpapillary thyroid cancersurgical molecular guidance

Identifiers

PMID26799257
PMCPMC4986916
OpenAlexW2234330084

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.