ArticlePloS one2016
Temporal Stability of the Salivary Microbiota in Oral Health.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
What it found
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Who cites it
96 citing papers in PubMed.
- The salivary microbiome is altered in the presence of a high salivary glucose concentration.PloS one · 2017Trial
- Multi-modality profiling identifies Neisseria flavescens as a central geroprotective oral commensal in humans.Nature aging · 2026Article
- Evaluating the Impact of Little Cigar Use on the Oral Bacterial Microbiota of Cigarette Smokers.Pathogens (Basel, Switzerland) · 2026Article
- The Role of Salivary Microbiota in Pancreatic Cancer: From Screening to Tumor Progression and Treatment Response.Biomedicines · 2026Review
- A generalizable cross-continent prediction of esophageal squamous cell carcinoma using the oral microbiome.Communications medicine · 2026Article
- Efficacy of very low calorie diet in the intervention of obesity and the changes in oral microbiota: a prospective cohort study.Journal of translational medicine · 2026Observational
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- Clinical translation of salivary MicroAge for understanding the oral-systemic connection in healthy longevity.Frontiers in microbiology · 2026Review
- Species-level taxonomic characterization enhances the power of saliva and feces stain microbiota for inferring the time since deposition (TsD).International journal of legal medicine · 2026Article
- Oral Microbiome in Oral Cancer Research from Sampling to Analysis: Strategies, Challenges, and Recommendations.Cancers · 2025Review
- A generalizable cross-continent prediction of esophageal squamous cell carcinoma using the oral microbiome.bioRxiv : the preprint server for biology · 2025Article
- Oral Bacterial and Fungal Microbiome and Subsequent Risk for Pancreatic Cancer.JAMA oncology · 2025Article
- Advanced Electrochemical Sensors for Rapid and Sensitive Monitoring of Tryptophan and Tryptamine in Clinical Diagnostics.Biosensors · 2025Review
- Gastric Cancer and Microbiota: Exploring the Microbiome's Role in Carcinogenesis and Treatment Strategies.Life (Basel, Switzerland) · 2025Review
- Article
- Review
- Circadian metabolic adaptations to infections.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Differential effects of high-fat diet on salivary and gut microbiota.Frontiers in cellular and infection microbiology · 2025Article
- Human Salivary Microbiota Diversity According to Ethnicity, Sex, TRPV1 Variants and Sensitivity to Capsaicin.International journal of molecular sciences · 2024Article
- Article
36 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
objectivesSaliva is a biological fluid suitable for biomarker analysis, and differences in the salivary microbiota in oral health and disease have been reported. For such comparative analyses, time of sampling is critical since the bacterial composition may vary throughout the day, i.e., diurnal variation. The purpose of this study is to compare the salivary microbiome over time to determine the optimal time for sampling.
designStimulated saliva samples were collected from 5 orally healthy individuals in 4 h intervals for 24 h, and collection was repeated 7 days later (number of samples per person, n = 12, total number of samples, n = 60). Salivary microbiota was analyzed using the Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS), and statistical analysis was performed using the Kruskal-Wallis test with Benjamini-Hochberg's correction for multiple comparisons, cluster analysis, principal component analysis and correspondence analysis.
resultsFrom a total of 60 saliva samples, 477 probe targets were collectively identified with a mean number of probes per sample of 207 (range: 153-307). Little or no variation in microbial profiles within subjects was observed over time.
conclusionsAlthough there was considerable variation between subjects, microbial profiles within subjects were stable throughout a 24 hour period and after 1 week. Since there is little or no evidence of diurnal variation of the salivary microbiome, time of sampling of saliva is not critical for perturbation or other microbial studies.
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