ArticleStructural dynamics (Melville, N.Y.)2015
Time-resolved structural studies with serial crystallography: A new light on retinal proteins.
Article in Structural dynamics (Melville, N.Y.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- Microsecond Time-Resolved Cryo-EM Based on Jet Vitrification.bioRxiv : the preprint server for biology · 2025Article
- Sample delivery methods for protein X-ray crystallography with a special focus on sample consumption.Nature communications · 2025Review
- Perspective: Structure determination of protein-ligand complexes at room temperature using X-ray diffraction approaches.Frontiers in molecular biosciences · 2023Article
- Rhodopsins: An Excitingly Versatile Protein Species for Research, Development and Creative Engineering.Frontiers in chemistry · 2022Review
- BL-11C Micro-MX: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at Pohang Light Source II.Journal of synchrotron radiation · 2021Article
- Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Opportunities and Challenges for Biosensors and Nanoscale Analytical Tools for Pandemics: COVID-19.ACS nano · 2020Review
- A temperature-controlled cold-gas humidifier and its application to protein crystals with the humid-air and glue-coating method.Journal of applied crystallography · 2019Article
- The Two-Photon Reversible Reaction of the Bistable Jumping Spider Rhodopsin-1.Biophysical journal · 2019Article
- SLS-2 - the upgrade of the Swiss Light Source.Journal of synchrotron radiation · 2018Article
- Perspective: Opportunities for ultrafast science at SwissFEL.Structural dynamics (Melville, N.Y.) · 2017Article
- Article
- Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography.Nature communications · 2016Article
- Moving beyond static snapshots: Protein dynamics and the Protein Data Bank.The Journal of biological chemistryReview
Corrections and comments
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Authors and funding
18 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Structural information of the different conformational states of the two prototypical light-sensitive membrane proteins, bacteriorhodopsin and rhodopsin, has been obtained in the past by X-ray cryo-crystallography and cryo-electron microscopy. However, these methods do not allow for the structure determination of most intermediate conformations. Recently, the potential of X-Ray Free Electron Lasers (X-FELs) for tracking the dynamics of light-triggered processes by pump-probe serial femtosecond crystallography has been demonstrated using 3D-micron-sized crystals. In addition, X-FELs provide new opportunities for protein 2D-crystal diffraction, which would allow to observe the course of conformational changes of membrane proteins in a close-to-physiological lipid bilayer environment. Here, we describe the strategies towards structural dynamic studies of retinal proteins at room temperature, using injector or fixed-target based serial femtosecond crystallography at X-FELs. Thanks to recent progress especially in sample delivery methods, serial crystallography is now also feasible at synchrotron X-ray sources, thus expanding the possibilities for time-resolved structure determination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.