ArticleStructural dynamics (Melville, N.Y.)2015
Real-time investigation of dynamic protein crystallization in living cells.
Article in Structural dynamics (Melville, N.Y.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Review
- Single-cell structural biology with intracellular electron crystallography.Nature communications · 2026Article
- Demixing of four simultaneously co-expressed phase-separating proteins in the endoplasmic reticulum lumen.Bioscience reports · 2025Article
- Intracellular protein crystallization in living insect cells.FEBS open bio · 2025Article
- Crowding and in-Cell Crystallization.Sub-cellular biochemistry · 2025Review
- Review
- Convolutional neural network approach for the automated identification ofJournal of applied crystallography · 2024Article
- A streamlined approach to structure elucidation using in cellulo crystallized recombinant proteins, InCellCryst.Nature communications · 2024Article
- Review of serial femtosecond crystallography including the COVID-19 pandemic impact and future outlook.Structure (London, England : 1993) · 2023Review
- Serial femtosecond crystallography.Nature reviews. Methods primers · 2022Article
- Article
- Molecular crowding and RNA synergize to promote phase separation, microtubule interaction, and seeding of Tau condensates.The EMBO journal · 2022Article
- Article
- Can (We Make)Toxins · 2021Review
- Correlation between Renal Artery Anatomy and Hypertension: A Retrospective Analysis of 3000 Patients.Evidence-based complementary and alternative medicine : eCAM · 2021Article
- Rapid screening ofJournal of applied crystallography · 2020Article
- Construction of gateway-compatible baculovirus expression vectors for high-throughput protein expression and in vivo microcrystal screening.Scientific reports · 2020Article
- Production and Isolation of Magnetic Protein Crystals in HEK293T Cells.Bio-protocol · 2020Article
- Enhanced X-ray diffraction of in vivo-grown μNS crystals by viscous jets at XFELs.Acta crystallographica. Section F, Structural biology communications · 2020Article
- In cellulo crystallization of Trypanosoma brucei IMP dehydrogenase enables the identification of genuine co-factors.Nature communications · 2020Article
Corrections and comments
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
X-ray crystallography requires sufficiently large crystals to obtain structural insights at atomic resolution, routinely obtained in vitro by time-consuming screening. Recently, successful data collection was reported from protein microcrystals grown within living cells using highly brilliant free-electron laser and third-generation synchrotron radiation. Here, we analyzed in vivo crystal growth of firefly luciferase and Green Fluorescent Protein-tagged reovirus μNS by live-cell imaging, showing that dimensions of living cells did not limit crystal size. The crystallization process is highly dynamic and occurs in different cellular compartments. In vivo protein crystallization offers exciting new possibilities for proteins that do not form crystals in vitro.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.